Anti-inflammatory secondary metabolites from Scrophularia kotschyana

• Published in: Human & experimental toxicology Vol. 40; no. 12_suppl; p. S676
• Main Authors: Renda, Gülin, Kadıoğlu, Mine, Kılıç, Merve, Korkmaz, Büşra, Kırmızıbekmez, Hasan
• Format: Journal Article
• Language: English
• Published: England 01-12-2021
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Mice; Mice, Inbred BALB C; Plant Extracts - pharmacology; Scrophularia - metabolism; Scrophularia kotschyana; flavonoid glycosides; anti-inflammatory activity; formalin test; iridoid glycosides
• ISSN: 1477-0903
• DOI: 10.1177/09603271211058889

The species belonging to
genus grow mainly in Irano-Turanian and Mediterranean regions and have been used as folk remedy for inflammatory-related diseases since ancient times. The present study was aimed to evaluate the anti-inflammatory activity of the extracts of
as well as the isolated compounds. The aerial parts and the roots of the plant were separately extracted with methanol. Anti-inflammatory activities of both extracts were evaluated with formalin test in mice. As the methanolic extract of the aerial parts significantly (
< .05) inhibited inflammation, it was then submitted to successive solvent extractions with
-hexane, dichloromethane, ethyl acetate and
-butanol to yield subextracts. Anti-inflammatory activities of the subextracts were evaluated within the same test system. Among the subextracts tested, the
-butanol subextract produced a significant (
< .05) anti-inflammatory activity at all doses (5, 10, and 30 mg/kg, ip.). Sequential chromatographic separation of the
-butanol subextract yielded 8-O-acetyl-4
-
-(
)-
-coumaroylharpagide, 8-
-acetyl-4
-
-(
)-
-coumaroylharpagide, β-sitosterol 3-
-β-glucopyranoside, apigenin 7-
-β-glucopyranoside, apigenin 7-
-rutinoside, luteolin 7-
-β-glucopyranoside and luteolin 7-
-rutinoside. The anti-inflammatory activities of the isolates were evaluated at 5 mg/kg dose. Luteolin 7-
-β-glucopyranoside and apigenin 7-
-rutinoside caused a significant (
< .05) inhibition of oedema formation.