Retinoic acid-inducible gene-I is induced by interferon-γ and regulates CXCL11 expression in HeLa cells

• Published in: Life sciences (1973) Vol. 82; no. 11; pp. 670 - 675
• Main Authors: Yuzawa, Ei, Imaizumi, Tadaatsu, Matsumiya, Tomoh, Yoshida, Hidemi, Fukuhara, Rie, Kimura, Hidetaka, Fukui, Atsushi, Tanji, Kunikazu, Mori, Fumiaki, Wakabayashi, Koichi, Fujii, Syunsaku, Mizunuma, Hideki, Satoh, Kei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 12-03-2008
• Subjects: Chemokine CXCL11 - genetics; Chemokine CXCL11 - metabolism; CXCL11; DEAD Box Protein 58; DEAD-box RNA Helicases - genetics; DEAD-box RNA Helicases - metabolism; Endometrium; Endometrium - cytology; Endometrium - metabolism; Female; Gene Expression Regulation; HeLa Cells; Humans; IFN-γ; Implantation; Interferon-gamma - metabolism; Pregnancy; Receptors, Immunologic; Reproduction; Retinoic acid-inducible gene-I; RNA Interference; Retinoic acid-inducible gene-I; Implantation; CXCL11; IFN-γ; Reproduction; Endometrium
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2007.12.025

Retinoic acid-inducible gene-I (RIG-I) is a member of the DExH box family proteins, which have diverse roles in the regulation of gene expression and cellular functions. RIG-I is one of the factors regulated by interferon (IFN)-γ and regarded as an intracellular signaling molecule for IFN-γ. IFN-γ is a major cytokine and also suggested to be involved in embryonal implantation and pregnancy. It is demonstrated that IFN-γ stimulates endometrial epithelial cells to produce CXCL11, which is implicated in implantation. The aim of the present study was to investigate the effect of IFN-γ on RIG-I expression in HeLa cells, a cell line derived from human uterine carcinoma. We found that RIG-I mRNA and protein were expressed in HeLa cells stimulated with IFN-γ. The effect of IFN-γ was observed in concentration- and time-dependent manners. The RNA interference against RIG-I resulted in the suppression of the IFN-γ-induced CXCL11 expression. Immunohistochemical studies revealed the RIG-I expression in the normal human endometrium, suggesting a possible role of RIG-I in human reproductive organs.