Development of Recombinant Proteins to Treat Chronic Pain

Chronic pain is difficult to treat and new approaches to resolve persistent pain are urgently needed. Anti-inflammatory cytokines are promising candidates for treating debilitating pain conditions due to their capacity to regulate aberrant neuro-immune interactions. However, physiologically they wor...

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Bibliographic Details
Published in:	Journal of visualized experiments no. 134
Main Authors:	Prado, Judith, Popov-Celeketic, Jelena, Steen-Louws, Cristine, Raoof, Ramin, Hack, Eric, Eijkelkamp, Niels
Format:	Journal Article
Language:	English
Published:	United States MyJove Corporation 11-04-2018
Subjects:	Animals Chronic Pain Female Humans Male Medicine Mice Recombinant Fusion Proteins - metabolism Transfection
Online Access:	Get full text
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Summary:	Chronic pain is difficult to treat and new approaches to resolve persistent pain are urgently needed. Anti-inflammatory cytokines are promising candidates for treating debilitating pain conditions due to their capacity to regulate aberrant neuro-immune interactions. However, physiologically they work in a network of various cytokines, and therefore their therapeutic effect may not be optimal when used as stand-alone drugs. To overcome this limitation, we developed a fusion protein of the anti-inflammatory cytokines IL4 and IL10. Here, we describe the methods for production and quality control of IL4-10 recombinant fusion protein and we test the effectiveness of the IL4-10 fusion protein to resolve pain in a mouse model of persistent inflammatory pain.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23 Correspondence to: Niels Eijkelkamp at N.Eijkelkamp@umcutrecht.nl
ISSN:	1940-087X 1940-087X
DOI:	10.3791/57071