DNA mismatch detection using a pyrene-excimer-forming probe

A pyrene-excimer-forming probe allowed the easy and sensitive detection of a single base mismatch in target DNA. This was due to the faster strand exchange rate compared to a fully-matched target.

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Bibliographic Details
Published in:	Chemical communications (Cambridge, England) no. 19; p. 2509
Main Authors:	Yamana, Kazushige, Fukunaga, Yudai, Ohtani, Yusuke, Sato, Sayaka, Nakamura, Mitsunobu, Kim, Won Jong, Akaike, Toshihiro, Maruyama, Atsushi
Format:	Journal Article
Language:	English
Published:	England 21-05-2005
Subjects:	Base Pair Mismatch DNA - chemistry DNA - genetics DNA Mutational Analysis - methods Pyrenes - chemistry Spectrometry, Fluorescence Thermodynamics
Online Access:	Get more information
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Description
Summary:	A pyrene-excimer-forming probe allowed the easy and sensitive detection of a single base mismatch in target DNA. This was due to the faster strand exchange rate compared to a fully-matched target.
ISSN:	1359-7345
DOI:	10.1039/b502033f