Overexpression of the trehalose-6-phosphate phosphatase OsTPP3 increases drought tolerance in rice

• Published in: Plant biotechnology reports Vol. 13; no. 3; pp. 285 - 292
• Main Authors: Jiang, Dagang, Chen, Weiting, Gao, Jieer, Yang, Fen, Zhuang, Chuxiong
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01-06-2019; Springer Nature B.V; 한국식물생명공학회
• Subjects: Abscisic acid; Agriculture; Biomedical and Life Sciences; Biosynthesis; Biotechnology; Cell Biology; Drought; Drought resistance; Life Sciences; Original Article; Oryza sativa; Plant Biochemistry; Plant Sciences; Plants (botany); Rice; Trehalose; Trehalose-6-phosphate; Trehalose-6-phosphate phosphatase; 농학; ABA; Trehalose; Drought tolerance; Rice
• ISSN: 1863-5466; 1863-5474
• DOI: 10.1007/s11816-019-00541-4

Trehalose plays an important role in mediating stress responses in plants, and trehalose-6-phosphate synthases and trehalose-6-phosphate phosphatases are essential for trehalose biosynthesis. Here, we address the function of rice (
Oryza sativa
)
OsTPP3
. We analyzed the expression of
OsTPP3
in different tissues and stress conditions, and generated
OsTPP3
-overexpressing rice plants. These plants showed a higher tolerance to simulated drought conditions (10% PEG treatment) than wild-type (WT) plants. Reverse-transcription quantitative PCR analysis indicated that transcript levels of genes related to stress responses and abscisic acid biosynthesis were significantly higher in the
OsTPP3
overexpressors than in WT plants. These results highlight the importance of
OsTPP3
in conferring drought tolerance in rice.