Changes in trophoblasts gene expression in response to perchlorate exposition

• Published in: Toxicology in vitro Vol. 50; pp. 328 - 335
• Main Authors: la Peña Sol, De, Isela, Santiago-Roque, Zendy, Olivo-Vidal, Mónica, Navarro-Meza, Irene, Xochihua-Rosas, Omar, Arroyo-Helguera
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-08-2018; Elsevier Science Ltd
• Subjects: ACE2; Angiotensin-converting enzyme 2; C/EBPβ; CCAAT/enhancer-binding protein; Chlorates; Copper; Cytotoxicity; DNA microarrays; Epidemiology; Epidermal growth factor receptors; Exposure; Gene expression; HIF1-α; Iodine; Iodine deficiency; Metal oxides; Migration; Nanomaterials; Nanoparticles; Nitric oxide; Nuclear transport; Oxidative stress; Perchlorate; Perchloric acid; Polymerase chain reaction; Potassium perchlorates; Pre-eclampsia; Protein transport; Proteins; Public health; Reactive oxygen species; Risk analysis; Risk factors; Translocation; Trophoblasts; Water pollution; C/EBPβ; Perchlorate; Oxidative stress; HIF1-α; Iodine deficiency; Nitric oxide
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/j.tiv.2018.04.006

Contaminated water with chlorates is a public health problem associated with iodine deficiency. Epidemiological evidence shows that iodine deficiency is a risk factor for preeclampsia (PE). In this study we use human BeWo trophoblast cells exposed to perchlorate (KClO4) and changes in gene expression were analyzed by microarrays, quantitative RT-PCR (qRT-PCR) and immunoblot. The microarray analysis identified 48 transcripts up-regulated and 112 down-regulated in comparison with non-exposed trophoblast. The qRT-PCR analysis confirmed changes in GAS7, PKP2, Emilin, Dynatic 3, protocadherins 11, 15, gamma A12, EGFR, SAFB1, ACE2, ANXA2, Apoliprotein E, SREBF1, and C/EBP-β. KClO4 exposition decreased the mRNA and protein of C/EBP-β and GPX4. Also, we observed a nuclear translocation of HIF1α protein, and increase in both Snail and ACE2 protein by immunoblot. These effects were accompanied by an increases in ROS and nitric oxide. In conclusion, our results show that exposure to KClO4 alters genes involved in migration, adhesion, differentiation, and correlate with the increase of oxidative stress and nitric oxide production in trophoblast cells. It is possible that iodine deficiency is associated with these processes. However, further studies are required to corroborate the role of iodine in trophoblast cells.
•Trophoblasts exposed to KClO4 increase reactive oxygen species and nitric oxide.•KClO4 alter the expression of 160 genes related to migration, differentiation and adhesion.•KClO4 decreases C/EBP-β and GPX4 proteins.•KCLO4 increases HIF1- α, Snail and ACE2 proteins.