In vitro exposure to pyrethroid-based products disrupts development of mouse preimplantation embryos

• Published in: Toxicology in vitro Vol. 57; pp. 184 - 193
• Main Authors: Babeľová, Janka, Šefčíková, Zuzana, Čikoš, Štefan, Kovaříková, Veronika, Špirková, Alexandra, Pisko, Jozef, Koppel, Juraj, Fabian, Dušan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-06-2019; Elsevier Science Ltd
• Subjects: Blastocysts; Cyclohexanone; Cyhalothrin; Degeneration; Deltamethrin; Developmental stages; Embryogenesis; Embryonic growth stage; Embryos; Fenvalerate; Impact damage; In vitro; Ingredients; Insecticides; Mouse; Organic chemistry; Permethrin; Preimplantation embryo; Pyrethroids; Toxicity; Pyrethroids; Preimplantation embryo; Mouse; In vitro
• ISSN: 0887-2333; 1879-3177
• DOI: 10.1016/j.tiv.2019.03.009

The objective of this study was to evaluate the potential toxicity of pyrethroids (deltamethrin, permethrin, fenvalerate, λ-cyhalothrin), commercial pyrethroid-based products DECIS EW 50 (deltamethrin mixture), TOP SPOT ON STRONGER (permethrin mixture), as well as related secondary ingredients on mouse preimplantation embryo development.
Two-cell stage embryos were in vitro cultured with addition of the listed chemicals until blastocyst formation. All active pyrethroids negatively affected embryonic development at 1000 μM concentration. Decreased quality of obtained blastocysts in permethrin, fenvalerate and λ-cyhalothrin-treated embryos was revealed as well. Deltamethrin showed harmful impact on embryo development at 100 μM concentration. Lower concentrations of pyrethroids (1, 10 μM) had no effect on embryo development.
The presence of DECIS EW 50 containing deltamethrin at 100 μM caused degeneration of all embryos. Similarly, TOP SPOT ON STRONGER containing 100 μM of permethrin impaired embryonic development and quality of obtained blastocysts. Evaluated secondary ingredients (butylhydroxyanisol, butylhydroxytoluen, butylparaben and cyclohexanone) at corresponding concentrations showed damaging impact on preimplantation embryo development as well.
Our results indicate that the embryotoxic potential of active pyrethroids is relatively low, whereas pyrethroid-based products have relatively high potential to impair mouse preimplantation development. Embryotoxicity of commercial products is probably attributable to the presence of secondary ingredients.
•Active pyrethroid substances showed embryotoxicity only at very high concentrations.•Pyrethroid products impaired mouse embryo growth in vitro even at low concentrations.•Secondary ingredients potentiated toxicity of TOP SPOT ON STRONGER and DECIS EW50.