Principles of 60S ribosomal subunit assembly emerging from recent studies in yeast

• Published in: Biochemical journal Vol. 474; no. 2; p. 195
• Main Authors: Konikkat, Salini, Woolford, Jr, John L
• Format: Journal Article
• Language: English
• Published: England 15-01-2017
• Subjects: Active Transport, Cell Nucleus; Binding Sites; Cell Nucleolus - genetics; Cell Nucleolus - metabolism; Cell Nucleolus - ultrastructure; Cytoplasm - genetics; Cytoplasm - metabolism; Cytoplasm - ultrastructure; Models, Molecular; Organelle Biogenesis; Protein Binding; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Interaction Domains and Motifs; Ribosomal Proteins - chemistry; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; Ribosome Subunits, Large, Eukaryotic - genetics; Ribosome Subunits, Large, Eukaryotic - metabolism; Ribosome Subunits, Large, Eukaryotic - ultrastructure; RNA Precursors - chemistry; RNA Precursors - genetics; RNA Precursors - metabolism; RNA, Ribosomal - chemistry; RNA, Ribosomal - genetics; RNA, Ribosomal - metabolism; RNA, Small Nucleolar - chemistry; RNA, Small Nucleolar - genetics; RNA, Small Nucleolar - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae - ultrastructure; ribosomes; ribonucleoproteins; ribosome assembly; yeast
• ISSN: 1470-8728
• DOI: 10.1042/BCJ20160516

Ribosome biogenesis requires the intertwined processes of folding, modification, and processing of ribosomal RNA, together with binding of ribosomal proteins. In eukaryotic cells, ribosome assembly begins in the nucleolus, continues in the nucleoplasm, and is not completed until after nascent particles are exported to the cytoplasm. The efficiency and fidelity of ribosome biogenesis are facilitated by >200 assembly factors and ∼76 different small nucleolar RNAs. The pathway is driven forward by numerous remodeling events to rearrange the ribonucleoprotein architecture of pre-ribosomes. Here, we describe principles of ribosome assembly that have emerged from recent studies of biogenesis of the large ribosomal subunit in the yeast Saccharomyces cerevisiae We describe tools that have empowered investigations of ribosome biogenesis, and then summarize recent discoveries about each of the consecutive steps of subunit assembly.