Effect of polyethylene glycol additives on structure, stability, and biocatalytic activity of ormosil sol–gel encapsulated yeast cells

• Published in: Journal of sol-gel science and technology Vol. 88; no. 1; pp. 1 - 5
• Main Authors: Lavrova, D. G., Kamanina, O. A., Machulin, A. V., Suzina, N. E., Alferov, V. A., Ponamoreva, O. N.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-10-2018; Springer Nature B.V
• Subjects: Additives; Biosensors; Ceramics; Chemical synthesis; Chemistry and Materials Science; Composites; Encapsulation; Glass; Inorganic Chemistry; Materials Science; Microscopy; Nanotechnology; Natural Materials; Optical and Electronic Materials; Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications; Phase contrast; Polyethylene glycol; Scanning electron microscopy; Sol-gel processes; Structural stability; Tetraethyl orthosilicate; Yeast; Encapsulated cells; ORMOSIL; PEG; sol–gel; Biosensor; Biocatalyst
• ISSN: 0928-0707; 1573-4846
• DOI: 10.1007/s10971-017-4333-z

Biohybrid materials based on ormosil encapsulated yeast cells were synthesized through a one-step sol–gel route with base-catalyst (NaF) using tetraethoxysilane (TEOS), methyltriethoxysilane (MTES) and polyethylene glycol (PEG) with different molar weights as a structure-controlling agent. Phase contrast microscopy and scanning electron microscopy were employed to evidence possible structures of the materials. The addition of PEG during cell encapsulation has induced structural changes within the biohybrids, which depend on PEG molecular weights. The biocatalytic activity of the living hybrids has been investigated by a biosensor which was based on the Clark-type oxygen electrode.
Graphical Abstract