TRP-2 specifically decreases WM35 cell sensitivity to oxidative stress

• Published in: Free radical biology & medicine Vol. 44; no. 6; pp. 1023 - 1031
• Main Authors: Michard, Quentin, Commo, Stephane, Belaidi, Jean-Philippe, Alleaume, Anne-Marie, Michelet, Jean-Francois, Daronnat, Edwige, Eilstein, Joan, Duche, Daniel, Marrot, Laurent, Bernard, Bruno Alain
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15-03-2008
• Subjects: Blotting, Western; Cell Line, Tumor; Chromatography, High Pressure Liquid; Comet Assay; DNA Damage - physiology; Dopachrome tautomerase; Epithelial Cells - metabolism; Glutathione; GSH; Humans; Immunohistochemistry; Intramolecular Oxidoreductases - metabolism; Melanocytes; Melanoma - metabolism; Oxidative stress; Oxidative Stress - physiology; RNA Interference; RNAi; Tandem Mass Spectrometry; TRP-2; PBS; Oxidative stress; RNAi; DHICA; NHEM; FRT; Melanocytes; Comet assay; TRP-2; TRP; DCT; FBS; ROS; Dopachrome tautomerase; GSH
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2007.11.021

TRP-2 (dopachrome tautomerase) is a melanogenic enzyme whose expression was recently reported to modulate melanocyte response to different cytotoxic events. Here we studied a possible role of TRP-2 in the oxidative stress response in the amelanotic WM35 melanoma cell line. Cell viability assays showed that TRP-2 overexpression in WM35 cells reduced their sensitivity to oxidative stress. Comet assays linked TRP-2 expression to DNA damage protection, and high-performance liquid chromotography—tandem mass spectrometry experiments showed an increase in intracellular glutathione in TRP-2-overexpressing cells. These effects were specifically reversed when TRP-2 was silenced by RNA interference. Nevertheless, these properties appeared to depend on a particular cell environment because expression of TRP-2 failed to rescue HEK epithelial cells exposed to similar treatments.