99mTc-HMPAO SPECT imaging reveals brain hypoperfusion during status epilepticus

• Published in: Metabolic brain disease Vol. 36; no. 8; pp. 2597 - 2602
• Main Authors: Bascuñana, Pablo, Wolf, Bettina J., Jahreis, Ina, Brackhan, Mirjam, García-García, Luis, Ross, Tobias L., Bengel, Frank M., Bankstahl, Marion, Bankstahl, Jens P.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-12-2021; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Blood flow; Brain; Brain damage; Brain mapping; Cognitive ability; Computed tomography; Convulsions & seizures; Epilepsy; Inflammation; Lithium; Medical imaging; Metabolic Diseases; Neural networks; Neuroimaging; Neurology; Neurosciences; Oncology; Oxygen; Oxygen requirement; Perfusion; Pilocarpine; Seizures; Short Communication; Single photon emission computed tomography; Neuroimaging; Pilocarpine; Brain perfusion; Epilepsy
• ISSN: 0885-7490; 1573-7365
• DOI: 10.1007/s11011-021-00843-z

Status epilepticus (SE) is a clinical emergency with high mortality. SE can trigger neuronal death or injury and alteration of neuronal networks resulting in long-term cognitive decline or epilepsy. Among the multiple factors contributing to this damage, imbalance between oxygen and glucose requirements and brain perfusion during SE has been proposed. Herein, we aimed to quantify by neuroimaging the spatiotemporal course of brain perfusion during and after lithium-pilocarpine-induced SE in rats. To this purpose, animals underwent
99m
Tc-HMPAO SPECT imaging at different time points during and after SE using a small animal SPECT/CT system.
99m
Tc-HMPAO regional uptake was normalized to the injected dose. In addition, voxel-based statistical parametric mapping was performed. SPECT imaging showed an increase of cortical perfusion before clinical seizure activity onset followed by regional hypo-perfusion starting with the first convulsive seizure and during SE. Twenty-four hours after SE, brain
99m
Tc-HMPAO uptake was widely decreased. Finally, chronic epileptic animals showed regionally decreased perfusion affecting hippocampus and cortical sub-regions. Despite elevated energy and oxygen requirements, brain hypo-perfusion is present during SE. Our results suggest that insufficient compensation of required blood flow might contribute to neuronal damage and neuroinflammation, and ultimately to chronic epilepsy generated by SE.