Emergence of rmtD1 gene in clinical isolates of Pseudomonas aeruginosa carrying blaKPC and/or blaVIM-2 genes in Brazil

• Published in: Brazilian journal of microbiology Vol. 52; no. 4; pp. 1959 - 1965
• Main Authors: Costa-Júnior, Sérgio Dias, da Silva, Adriana Maria Costa Marques, Niedja da Paz Pereira, Jussyêgles, da Costa Lima, Jailton Lobo, Cavalcanti, Isabella Macário Ferro, Maciel, Maria Amélia Vieira
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-12-2021; Springer Nature B.V
• Subjects: Aminoglycosides; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Biomedical and Life Sciences; Carbapenemase; Clinical isolates; Clinical Microbiology - Research Paper; Epidemiology; Food Microbiology; Genes; Life Sciences; Medical Microbiology; Methylase; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Mycology; Nosocomial infection; Polymyxin B; Pseudomonas aeruginosa; rRNA (adenosine-2'-0'-)-methyltransferase; rRNA 16S; Sequences; Tobramycin; Brazil; Clonal dissemination; Carbapenemases; 16S rRNA methylases
• ISSN: 1517-8382; 1678-4405
• DOI: 10.1007/s42770-021-00576-2

Objectives
The aim of the present study is to describe clinical aminoglycoside- or carbapenem-resistant
Pseudomonas aeruginosa
isolates collected between 2018 and 2019 in a hospital in Recife City, Northeastern Brazil. It was done based on phenotypic and molecular markers of antimicrobial resistance, as well as on the clonal diversity of the investigated isolates.
Methods
Thirty-four carbapenem- and/or aminoglycoside-resistant
P. aeruginosa
isolates were collected in a hospital in Recife City-PE, Brazil. Their antimicrobial susceptibility profile was identified based on the automated BD Phoenix ™ system. In addition, broth microdilution was performed to determine the MICs of tobramycin and polymyxin B. Eventually, isolates were subjected to PCR and sequencing in order to detect the carbapenemase enzyme (
bla
KPC
,
bla
NDM
,
bla
VIM
,
bla
SPM-1
, and
bla
IMP
) and 16S rRNA methylase (
armA
,
rmtB
,
rmtD
,
rmtF
, and
rmtG
) genes; ERIC-PCR was conducted for clonal profile determination purposes.
Results
Thirty-four of the 64 isolates evaluated in the present study were selected for complementary molecular phenotypic tests, based on sample inclusion criteria. The
bla
KPC
and
bla
VIM-2
genes were identified in 32.4% (11/34) and 38.2% (13/34) of tested isolates, respectively. The
rmtD1
gene was detected in 32.4% (11/34) of analyzed isolates. Eight isolates carried both the
bla
KPC
and
rmtD1
genes, whereas
bla
VIM-2
and
rmtD1
genes co-occurrence was detected in three strains; one isolate had all
bla
KPC
,
bla
VIM-2
, and
rmtD1
genes. ERIC-PCR molecular typing has evidenced cross-transmission of three pathogenic clones among patients in the hospital.
Conclusions
The present study is a pioneer in describing isolates harboring both
bla
VIM-2
and
rmtD1
genes. Moreover, it emphasizes the need of conducting local molecular epidemiology studies at different time intervals in order to monitor measures adopted to prevent nosocomial infections in different hospital units.