Large scale, prospective screening of EGFR mutations in the blood of advanced NSCLC patients to guide treatment decisions

• Published in: Annals of oncology Vol. 28; no. 9; pp. 2248 - 2255
• Main Authors: Mayo-de-las-Casas, C., Jordana-Ariza, N., Garzón-Ibañez, M., Balada-Bel, A., Bertrán-Alamillo, J., Viteri-Ramírez, S., Reguart, N., Muñoz-Quintana, M.A., Lianes-Barragan, P., Camps, C., Jantús, E., Remon-Massip, J., Calabuig, S., Aguiar, D., Gil, M.L., Viñolas, N., Santos-Rodríguez, A.K., Majem, M., García-Peláez, B., Villatoro, S., Pérez-Rosado, A., Monasterio, J.C., Ovalle, E., Catalán, M.J., Campos, R., Morales-Espinosa, D., Martínez-Bueno, A., González-Cao, M., González, X., Moya-Horno, I., Sosa, A.E., Karachaliou, N., Rosell, R., Molina-Vila, M.A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-09-2017
• Subjects: Adult; Aged; Carcinoma, Non-Small-Cell Lung - drug therapy; Carcinoma, Non-Small-Cell Lung - genetics; Decision Making; Drug Resistance, Neoplasm - genetics; EGFR mutations; ErbB Receptors - antagonists & inhibitors; ErbB Receptors - genetics; Female; Genetic Testing; Humans; liquid biopsy; Lung Neoplasms - drug therapy; Lung Neoplasms - genetics; Male; Middle Aged; Mutation; NSCLC; Prospective Studies; Protein Kinase Inhibitors - therapeutic use; Real-Time Polymerase Chain Reaction; Treatment Outcome; tyrosine-kinase inhibitors (TKIs); tyrosine-kinase inhibitors (TKIs); EGFR mutations; NSCLC; liquid biopsy
• ISSN: 0923-7534; 1569-8041
• DOI: 10.1093/annonc/mdx288

In a significant percentage of advanced non-small-cell lung cancer (NSCLC) patients, tumor tissue is unavailable or insufficient for genetic analyses. We prospectively analyzed if circulating-free DNA (cfDNA) purified from blood can be used as a surrogate in this setting to select patients for treatment with epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs).
Blood samples were collected in 119 hospitals from 1138 advanced NSCLC patients at presentation (n = 1033) or at progression to EGFR-TKIs (n = 105) with no biopsy or insufficient tumor tissue. Serum and plasma were sent to a central laboratory, cfDNA purified and EGFR mutations analyzed and quantified using a real-time PCR assay. Response data from a subset of patients (n = 18) were retrospectively collected.
Of 1033 NSCLC patients at presentation, 1026 were assessable; with a prevalence of males and former or current smokers. Sensitizing mutations were found in the cfDNA of 113 patients (11%); with a majority of females, never smokers and exon 19 deletions. Thirty-one patients were positive only in plasma and 11 in serum alone and mutation load was higher in plasma and in cases with exon 19 deletions. More than 50% of samples had <10 pg mutated genomes/µl with allelic fractions below 0.25%. Patients treated first line with TKIs based exclusively on EGFR positivity in blood had an ORR of 72% and a median PFS of 11 months. Of 105 patients screened after progression to EGFR-TKIs, sensitizing mutations were found in 56.2% and the p.T790M resistance mutation in 35.2%.
Large-scale EGFR testing in the blood of unselected advanced NSCLC patients is feasible and can be used to select patients for targeted therapy when testing cannot be done in tissue. The characteristics and clinical outcomes to TKI treatment of the EGFR-mutated patients identified are undistinguishable from those positive in tumor.