Genome Variability in Artificial Allopolyploid Hybrids of Avena sativa L. and Avena macrostachya Balansa ex Coss. et Durieu Based on Marker Sequences of Satellite DNA and the ITS1-5.8S rDNA Region

• Published in: International journal of molecular sciences Vol. 25; no. 10; p. 5534
• Main Authors: Amosova, Alexandra V, Gnutikov, Alexander A, Rodionov, Alexander V, Loskutov, Igor G, Nosov, Nikolai N, Yurkevich, Olga Yu, Samatadze, Tatiana E, Zoshchuk, Svyatoslav A, Muravenko, Olga V
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19-05-2024
• Subjects: allopolyploid genome composition; Avena - classification; Avena - genetics; Avena macrostachya; Avena sativa; Barley; Breeding; Chromosomes; Chromosomes, Plant; DNA, Plant - genetics; DNA, Ribosomal - genetics; DNA, Satellite - genetics; Genes; Genetic Variation; Genomes; Hybridization; Hybridization, Genetic; interspecific hybridization; Kinases; Phylogeny; Polymorphism; ribotypes; Avena sativa; Avena macrostachya; ribotypes; allopolyploid genome composition; interspecific hybridization
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25105534

Artificial hybrids between cultivated
species and wild
that possess genes for resistance to biotic and abiotic stresses can be important for oat breeding. For the first time, a comprehensive study of genomes of artificial fertile hybrids
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and their parental species was carried out based on the chromosome FISH mapping of satellite DNA sequences (satDNAs) and also analysis of intragenomic polymorphism in the 18S-ITS1-5.8S rDNA region, using NGS data. Chromosome distribution patterns of marker satDNAs allowed us to identify all chromosomes in the studied karyotypes, determine their subgenomic affiliation, and detect several chromosome rearrangements. Based on the obtained cytogenomic data, we revealed differences between two
subgenomes and demonstrated that only one of them was inherited in the studied octoploid hybrids. Ribotype analyses showed that the second major ribotype of
was species-specific and was not represented in rDNA pools of the octoploids, which could be related to the allopolyploid origin of this species. Our results indicate that the use of marker satDNAs in cytogenomic studies can provide important data on genomic relationships within
allopolyploid species and hybrids, and also expand the potential for interspecific crosses for breeding.