Specific Globin mRNAs in Human Erythroleukemia (K562) Cells

• Published in: Blood Vol. 63; no. 1; pp. 195 - 200
• Main Authors: Miller, Carl W., Young, Katherine, Dumenil, Domnique, Alter, Blanche P., Schofield, J.Matthew, Bank, Arthur
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-01-1984
• Subjects: Cell Line; Globins - analysis; Globins - biosynthesis; Globins - genetics; Hemin - physiology; Humans; Leukemia, Erythroblastic, Acute - analysis; Leukemia, Erythroblastic, Acute - blood; Leukemia, Erythroblastic, Acute - genetics; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; RNA, Neoplasm - analysis; RNA, Neoplasm - biosynthesis; Transcription, Genetic
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V63.1.195.195

Specific globin mRNA accumulation was quantitated in several lines of K562 cells in the absence and the presence of hemin. Using specific cloned DNA probes, the amounts of ζ, α ∊ and γ mRNAs were shown to be increased 2-3-fold in the presence of 20 μM hemin. No δ- or β-globin mRNAs were detectable in any of the lines. In one line, Bos, there was a marked decrease in ∊-globin mRNA, which increased with hemin, although still to much lower levels than in the other lines. The decreased ∊-globin mRNA accumulation in Bos is shown to be due to decreased ∊-globin gene transcription.