In vivo developmental modifications of the expression of genes encoding muscle-specific enzymes in rat

• Published in: The Journal of biological chemistry Vol. 261; no. 22; pp. 10271 - 10276
• Main Authors: Schweighoffer, F, Maire, P, Tuil, D, Gautron, S, Daegelen, D, Bachner, L, Kahn, A
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 05-08-1986; American Society for Biochemistry and Molecular Biology
• Subjects: Aging; Animals; Biological and medical sciences; DNA - genetics; DNA - isolation & purification; Fetus - metabolism; Fructose-Bisphosphate Aldolase - metabolism; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation; Molecular and cellular biology; Molecular genetics; Muscle Development; Muscles - embryology; Muscles - enzymology; Nucleic Acid Hybridization; Phosphorylases - genetics; Poly A - metabolism; Rats; RNA - metabolism; RNA, Messenger - metabolism; Rat; Creatine kinase; Enzyme; Rodentia; Gene expression; Striated muscle; Phosphorylase; In vivo; Vertebrata; Regulation(control); Mammalia; Messenger RNA; Animal; Development
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(18)67519-2

cDNA clones for rat muscle-type creatine kinase and glycogen phosphorylase and aldolase A were isolated from a rat muscle cDNA library. An additional clone recognizing an unidentified 2.7-kilobase pair mRNA species was also isolated. These cDNA clones were used as probes to investigate the expression of the corresponding mRNAs during muscle development. Two aldolase A mRNA species were detected, one of 1650 bases expressed in non-muscle tissues, fetal muscle, and adult slow-twitch muscle, the other of 1550 bases was highly specific of adult fast-twitch skeletal muscle differentiation. These aldolase A mRNAs were shown by primer extension to differ by their 5' ends. The accumulation of muscle-type phosphorylase and creatine kinase and muscle-specific aldolase A mRNA accumulation during muscle development seems to be a coordinate process occurring progressively from the 17th day of intrauterine life up to the 30th day after birth. In contrast, the 2.7-kilobase pair RNA species is maximally expressed at the 1st week after birth as is the neonatal form of myosin heavy chain mRNA.