Five methods for detection of Helicobacter pylori in the Turkish population

• Published in: World journal of gastroenterology : WJG Vol. 17; no. 47; pp. 5172 - 5176
• Main Authors: Aktepe, Orhan Cem, Ciftçi, Ihsan Hakkı, Safak, Birol, Uslan, Ihsan, Dilek, Fatma Hüsniye
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Co., Limited 21-12-2011
• Subjects: Adult; Antigens, Bacterial - analysis; Brief; Diagnostic Tests, Routine - economics; Diagnostic Tests, Routine - methods; Diagnostic Tests, Routine - standards; Feces - microbiology; Helicobacter Infections - diagnosis; Helicobacter Infections - microbiology; Helicobacter pylori - genetics; Helicobacter pylori - immunology; Humans; In Situ Hybridization, Fluorescence - methods; Polymerase Chain Reaction - methods; Turkey; Turkey; Polymerase chain reaction; Helicobacter pylori stool antigen; Histology; Helicobacter pylori; Fluorescence in situ hybridization
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v17.i47.5172

AIM： To compare culture analysis, Helicobacter pylori （H. pylori） stool antigen （HpSA） test, polymerase chain reaction （PCR） and fluorescence in situ hybridization （FISH） for H. pylori detection. METHODS： One hundred and thirty-two consecutive adult dyspeptic patients receiving diagnostic endoscopy at the department of gastroenterology were enrolled in this study. Culture and histological examination were performed on biopsy specimens. PCR and FISH tests were applied to histopathological samples. Stool samples that were simultaneously collected were tested for the H. pylori antigen using the HpSA test and bacterial DNA using stool PCR. RESULTS： H. pylori was positively identified by histo-logical examination in 85/132 （64.4%） of the patients, while positive samples were found in 56 （42.4%）, 64 （48.5%）, 98 （74.2%）, 28 （21.2%） and 81 （61.4%） of the patients by culture, HpSA, PCR, stool PCR and FISH methods, respectively. The results of the culture, biopsy PCR, HpSA and FISH tests, with the exception of the stool PCR, were found to correlate with the histological examination as a gold standard. CONCLUSION： The HpSA test is a rapid, simple, and noninvasive test for monitoring therapy. FISH is an accurate, rapid, cost-effective, and easy-to-use test for H. pylori detection.