Occurrence of black rot of cultivated mushrooms (Flammulina velutipes) caused by Pseudomonas tolaasii in Korea

• Published in: European journal of plant pathology Vol. 133; no. 3; pp. 527 - 535
• Main Authors: Han, Hye-Su, Jhune, Chang-Sung, Cheong, Jong-Chun, Oh, Jin-A, Kong, Won-Sik, Cha, Jae-Soon, Lee, Chan-Jung
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-07-2012; Springer; Springer Nature B.V
• Subjects: Agriculture; Bacteria; Basidiocarps; Biochemical tests; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Black rot; Black spot; Ecology; Fatty acids; Flammulina velutipes; Fundamental and applied biological sciences. Psychology; Gram stain; Inoculation; Lesions; Life Sciences; lipopeptides; Mushrooms; Pathogenicity; Pathogens; Phytopathology. Animal pests. Plant and forest protection; Plant diseases; Plant Pathology; Plant Sciences; Polymerase chain reaction; Pseudomonas tolaasii; RpoB protein; rRNA 16S; Stipes; Asia; Far east; Korea; Pathogenicity; Black rot; Biochemical tests; gene; Fatty acids; Lipopeptide; 16S rRNA gene; Pseudomonadales; Agaricus bisporus; Plant pathogen; Ribosomal DNA; rpoB gene; Lipids; Flammulina velutipes; Basidiomycota; Fungi; Plant; Gene; 16S-RNA; Bacteria; Pseudomonadaceae; Pseudomonas tolaasii; Edible fungi
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1007/s10658-012-9941-4

From black spots on winter mushroom (
Flammulina velutipes
), fluorescent bacteria were repeatedly isolated during surveys at places of production in the years 2009–2010 in Korea. From these lesions three bacterial strains (designated CHM13, CHM16, CHM17) were isolated which, following inoculation of mushroom stipes and caps, yielded characteristic black spots and sunken lesions, which developed into a severe black rot. Results of Gram stain and biochemical tests preliminarily identified these isolates as
Pseudomonas tolaasii
. This was confirmed by pathogenicity to winter mushroom, physiological and biochemical properties, analysis of the 16S rRNA and
rpoB
gene sequences, fatty acids profile, specific and sensitive PCR assays and, lipopeptide detection. This is the first report of the isolation of
Pseudomonas tolaasii
from cultivated winter mushroom in Korea.