Complete nucleotide sequence of spring beauty latent virus, a bromovirus infectious to Arabidopsis thaliana

• Published in: Archives of virology Vol. 148; no. 1; pp. 165 - 175
• Main Authors: Fujisaki, K, Hagihara, F, Kaido, M, Mise, K, Okuno, T
• Format: Journal Article
• Language: English
• Published: Austria Springer-Verlag 2003; Springer Nature B.V
• Subjects: Arabidopsis - virology; Arabidopsis thaliana; Base Sequence; Broad bean mottle virus; Brome mosaic virus; Bromovirus - genetics; Bromovirus - pathogenicity; clones; complementary DNA; Cowpea chlorotic mottle virus; Genes, Viral; intergenic DNA; Molecular Sequence Data; nucleotide sequences; Open Reading Frames; Plant Diseases - virology; Plant Viruses - classification; Plant Viruses - genetics; RNA; RNA, Viral - genetics; Sequence Alignment; sequence analysis; Spring beauty latent virus
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-002-0909-0

Spring beauty latent virus (SBLV), a bromovirus, systemically and efficiently infected Arabidopsis thaliana, whereas the well-studied bromoviruses brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV) did not infect and poorly infected A. thaliana, respectively. We constructed biologically active cDNA clones of SBLV genomic RNAs and determined their complete nucleotide sequences. Interestingly, SBLV RNA3 contains both the box B motif in the intercistronic region, as does BMV, and the subgenomic promoter-like sequence in the 5’ noncoding region, as does CCMV. Sequence comparisons of SBLV, BMV, CCMV, and broad bean mottle virus demonstrated that SBLV is closely related to BMV and CCMV.