Antibacterial and osteogenic properties of silver-containing hydroxyapatite coatings produced using a sol gel process

• Published in: Journal of Biomedical Materials Research Part B Vol. 82A; no. 4; pp. 899 - 906
• Main Authors: Chen, W., Oh, S., Ong, A.P., Oh, N., Liu, Y., Courtney, H.S., Appleford, M., Ong, J.L.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 15-09-2007
• Subjects: Alkaline Phosphatase - metabolism; Anti-Bacterial Agents - pharmacology; bacterial adhesion; Bacterial Adhesion - drug effects; Cell Differentiation - drug effects; Cell Line; Cell Proliferation - drug effects; Coated Materials, Biocompatible; Durapatite - pharmacology; Embryonic Stem Cells - cytology; Embryonic Stem Cells - drug effects; Embryonic Stem Cells - enzymology; Gels; Humans; hydroxyapatite; Materials Testing; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - enzymology; osteoblast cell differentiation; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - enzymology; Osteogenesis - drug effects; Prostheses and Implants; silver; Silver - pharmacology; sol gel; Staphylococcus aureus - drug effects; Staphylococcus epidermidis - drug effects; X-Ray Diffraction
• ISSN: 1549-3296; 1552-4965; 1552-4981
• DOI: 10.1002/jbm.a.31197

Since bacterial infection is a rising complication following the wide use of implant, there is considerable attention on the effect of implant surface properties on bacterial adhesion. In this study, the effect of silver (Ag) doped hydroxyapatite (HA) coatings on initial antibacterial adhesion and osteoblast cell proliferation and differentiation was investigated. Using a sol‐gel process, HA coatings doped with 1 wt % AgNO3 (AgHA1.0) and 1.5 wt % Ag (AgHA1.5) were prepared. Coated surfaces were characterized using X‐ray diffraction (XRD) and contact angles measurements. The initial bacteria adhesion was evaluated using a RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus, whereas osteoblast proliferation and differentiation were evaluated using human embryonic palatal mesenchyme cells (HEPM), an osteoblast precursor cell line. In this study, XRD analysis of all surfaces indicated peaks corresponding to HA. Contact angles for AgHA surfaces were observed to be significantly lower when compared to HA surfaces. In vitro initial bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on AgHA surfaces when compared to HA surface. The use of HEPM cells indicated no significant difference in double‐stranded DNA (dsDNA) production between all surfaces. Additionally, no differences in alkaline phosphatase specific activity were observed between HA and AgHA1.0 surfaces. Overall, it was concluded that AgHA1.0 has the similar biological activity as HA, with respect to bone cell proliferation and differentiation. In addition, the AgHA1.0 was also concluded to have the ability to minimize the initial bacteria adhesion. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007