In vitro study of Seraspenide on HIV-induced inhibition of granulopoiesis

• Published in: European journal of haematology Vol. 59; no. 3; pp. 184 - 189
• Main Authors: Coutton, C., Chermann, J. C.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-09-1997; Blackwell
• Subjects: Action of physical and chemical agents; AIDS/HIV; Antigens, CD34 - analysis; Biological and medical sciences; CD34+ cells; Cell Differentiation - drug effects; Cells, Cultured; Culture Media, Conditioned - pharmacology; Fundamental and applied biological sciences. Psychology; Granulocytes - drug effects; granulopoiesis; Growth Inhibitors - pharmacology; Hematopoiesis - drug effects; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - virology; HIV; HIV Infections - immunology; Humans; Leukocytes, Mononuclear - metabolism; Leukocytes, Mononuclear - virology; Microbiology; Oligopeptides - pharmacology; Seraspenide; T-Lymphocyte Subsets - chemistry; Virology; Human; Leukopoiesis; Conditioned medium; Retroviridae; In vitro; Virus; Prevention; Tetrapeptide; Hematopoiesis; Lentivirinae; Inhibition; Human immunodeficiency virus; Granulopoiesis
• ISSN: 0902-4441; 1600-0609
• DOI: 10.1111/j.1600-0609.1997.tb00973.x

Soluble(s) factor(s) produced by HIV‐infected cells have been implicated in bone marrow dysfunction observed in AIDS patients. We have shown previously that infected macrophage but not CBMC‐derived conditioned medium inhibited the differentiation of purified CD34+ cells, suggesting that accessory cells play a crucial role in granulopoiesis inhibition induced indirectly by HIV. In order to prevent this inhibition we tested the potential protective effect of Seraspenide, a reversible inhibitor of hematopoietic progenitors. We showed that 20 h pretreatment of mononuclear cells with Seraspenide at 10−10 mol/1 prevents CFU‐GM inhibition induced by HIV‐infected cell‐derived conditioned medium.