Methylenetetrahydrofolate reductase gene polymorphisms are associated with ischemic and hemorrhagic stroke: Dual effect of MTHFR polymorphisms C677T and A1298C

• Published in: Brain research bulletin Vol. 71; no. 1; pp. 45 - 50
• Main Authors: Sazci, Ali, Ergul, Emel, Tuncer, Nese, Akpinar, Gurler, Kara, Ihsan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 11-12-2006
• Subjects: Adult; Aged; Aged, 80 and over; Association; Brain Ischemia - genetics; Brain Ischemia - metabolism; Brain Ischemia - physiopathology; DNA Mutational Analysis; European Continental Ancestry Group; Female; Gene Frequency; Genetic Markers - genetics; Genetic Predisposition to Disease - genetics; Genetic Testing; Genotype; Hemorrhagic stroke; Humans; Intracranial Hemorrhages - genetics; Intracranial Hemorrhages - metabolism; Intracranial Hemorrhages - physiopathology; Ischemic stroke; Male; Methylenetetrahydrofolate Reductase (NADPH2) - genetics; Middle Aged; MTHFR; Mutation - genetics; Polymorphism; Polymorphism, Genetic - genetics; Stroke - genetics; Stroke - metabolism; Stroke - physiopathology; Turkey; Turkey; MTHFR; Association; Turkey; Ischemic stroke; Hemorrhagic stroke; Polymorphism
• ISSN: 0361-9230; 1873-2747
• DOI: 10.1016/j.brainresbull.2006.07.014

Hyperhomocysteinemia is an independent risk factor for ischemic stroke. The enzyme methylenetetrahydrofolate reductase (MTHFR) plays a critical role in modulating the levels of plasma homocysteine. Two polymorphisms in the MTHFR gene, C677T, A1298C result in reduced enzyme activity. The mechanisms of ischemic and hemorrhagic stroke are not well understood. Although controversial, previous studies have shown evidence of causality of both stroke subtypes in patients with methylenetetrahydrofolate reductase gene polymorphisms. Therefore, we examined whether the C677T and A1298C polymorphisms of MTHFR gene are genetic risk factors for both ischemic and hemorrhagic stroke in a Turkish Caucasian population. In a case–control study, 120 total unrelated stroke patients (92 ischemic stroke, 28 hemorrhagic stroke), and 259 healthy controls were genotyped for C677T and A1298C polymorphisms of the MTHFR gene using a PCR-RFLP based-method. The MTHFR 1298C allele (
χ
2
=
8.589;
P
=
0.014), C1298C genotype (OR
=
2.544;
P
=
0.004), and C677C/C1298C compound genotype (OR
=
3.020;
P
=
0.001) were associated with overall stroke. The MTHFR 1298C allele (
χ
2
=
11.166;
P
=
0.004), C1298C genotype (OR
=
2.950;
P
=
0.001), and C677C/C1298C compound genotype (OR
=
3.463,
P
=
0.0001) were strongly associated with ischemic stroke. Interestingly however, the MTHFR 677T allele (
χ
2
=
6.033;
P
=
0.049), T677T genotype (OR
=
3.120;
P
=
0.014), and T677T/A1298A compound genotype (OR
=
4.211;
P
=
0.002) were associated with hemorrhagic stroke. In conclusion, the C677T and A1298C polymorphisms of the MTHFR gene are genetic risk factors for hamorrhagic and ischemic stroke respectively, independent of other atherothrombotic risk factors.