Simplified sequence-specific oligonucleotide-based polymerase chain reaction protocol to characterize human major histocompatibility complex A02 and A24 specificities

• Published in: Human immunology Vol. 72; no. 10; pp. 869 - 871
• Main Authors: Ferrando-Martinez, Sara, Leal, Manuel, González-Escribano, María Francisca, Vega, Yolanda, Ruiz-Mateos, Ezequiel
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-10-2011
• Subjects: Alleles; Allergy and Immunology; DNA Primers - chemistry; DNA Primers - genetics; Gene Frequency; Histocompatibility Testing - methods; HLA; Humans; Major Histocompatibility Complex - genetics; Major Histocompatibility Complex - immunology; MHC; Molecular Typing - methods; Multimers; PCR; Peptides - analysis; Peptides - chemistry; Peptides - immunology; Polymerase Chain Reaction; Protein Multimerization - genetics; Protein Multimerization - immunology; Sensitivity and Specificity; T-Lymphocytes - chemistry; T-Lymphocytes - cytology; T-Lymphocytes - immunology; Tetramers; MHC; Tetramers; HLA; PCR; Multimers
• ISSN: 0198-8859; 1879-1166
• DOI: 10.1016/j.humimm.2011.05.025

Abstract Major histocompatibility complex (MHC) multimer technology is used in studies of high scientific and clinical interest for the identification, analysis, purification, and adoptive transfer of virus-specific T cells. MHC peptide multimers are usually specific for MHC A*02 or A*24 specificities because both specificities exhibit a high worldwide frequency. However, commercially available typing methods perform complete typing instead of browsing for these prevailing specificities. In this study we demonstrate an easy and accessible polymerase chain reaction-based method to accurately identify A*02 and A*24 samples in a cost-effective way.