An impedimetric approach for COVID-19 detection

• Published in: Analyst (London) Vol. 147; no. 1; p. 130
• Main Authors: Tepeli Büyüksünetçi, Yudum, Çitil, Burak Ekrem, Anık, Ülkü
• Format: Journal Article
• Language: English
• Published: England 20-12-2021
• Subjects: COVID-19; Diagnostic Tests, Routine; Humans; SARS-CoV-2
• ISSN: 1364-5528
• DOI: 10.1039/d1an01718g

In this study, an electrochemical approach for the determination of coronavirus disease (COVID-19) was developed. The biosensor system relied on the spike protein (S-protein) based infection mechanism of the virus and included separate interactions of receptors like angiotensin-converting enzyme 2 (ACE2) and CD147. After the optimization of experimental parameters, the analytical characteristics of both receptors ACE2 and CD147 were investigated. For ACE2 receptor, the linear detection ranges of the S-protein were found in the range of 700 ng mL
to 1500 ng mL
and from 1500 ng mL
to 7000 ng mL
with a limit of detection (LOD) value of 299.30 ng mL
. Meanwhile, for CD147 receptor the linear range was in the range of 500 ng mL
to 5000 ng mL
with a LOD value of 38.99 ng mL
. After the examination of analytical characteristics, the developed electrochemical approach was applied for severe acute respiratory syndrome coronavirus 2 samples and the obtained results were validated with real time polymerase chain reaction method.