Genome-wide identification of the grapevine β-1,3-glucanase gene (VviBG) family and expression analysis under different stresses

• Published in: BMC plant biology Vol. 24; no. 1; pp. 911 - 14
• Main Authors: Wang, Ling, Li, Ruilong, Li, Kaiwei, Qu, Ziyang, Zhou, Ruijin, Lu, Guilong, Li, Peng, Li, Guirong
• Format: Journal Article
• Language: English
• Published: England BioMed Central 01-10-2024; BMC
• Subjects: Abiotic stress; Airborne microorganisms; Bioinformatics; Chromosomes; Cold treatment; Cotton; Downy mildew; Drought; Expression pattern; Fruits; Fungi; Gene clusters; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genome, Plant; Genomes; Glucan 1,3-beta-Glucosidase - genetics; Glucan 1,3-beta-Glucosidase - metabolism; Grapes; Grapevine; Grapevines; Growth hormones; Hormones; Identification; Localization; Multigene Family; Pathogens; Phylogenetics; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Proteins; Sodium chloride; Stress, Physiological - genetics; Stresses; Subgroups; Synteny; Tobacco; Transcriptomes; Vitis - enzymology; Vitis - genetics; β-1,3-glucanase gene family; China; Abiotic stress; Expression pattern; Grapevine; β-1,3-glucanase gene family
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05597-1

The β-1,3-glucanase gene is widely involved in plant development and stress defense. However, an identification and expression analysis of the grape β-1,3-glucanase gene (VviBG) family had not been conducted prior to this study.
Here, 42 VviBGs were identified in grapevine, all of which contain a GH-17 domain and a variable C-terminal domain. VviBGs were divided into three clades α, β and γ, and six subgroups A-F, with relatively conserved motifs/domains and intron/exon structures within each subgroup. The VviBG gene family contained four tandem repeat gene clusters. There were intra-species synteny relationships between two pairs of VviBGs and inter-species synteny relationships between 20 pairs of VviBGs and AtBGs. The VviBG promoter contained many cis-acting elements related to stress and hormone responses. Tissue-specific analysis showed that VviBGs exhibited distinct spatial and temporal expression patterns. Transcriptome analysis indicated that many VviBGs were induced by wounds, UV, downy mildew, cold, salt and drought, especially eight VviBGs in subgroup A of the γ clade. RT-qPCR analysis showed that these eight VviBGs were induced under abiotic stress (except for VviBG41 under cold stress), and most of them were induced at higher expression levels by PEG6000 and NaCl than under cold treatment.
The chromosome localization, synteny and phylogenetic analysis of the VviBG members were first conducted. The cis-acting elements, transcriptome data and RT-qPCR analysis showed that VviBG genes play a crucial role in grape growth and stress (hormone, biotic and abiotic) responses. Our study laid a foundation for understanding their functions in grape resistance to different stresses.