Anti-Fatigue Activity of a Mixture of Stauntonia hexaphylla (Thunb.) Decaisne and Vaccinium bracteatum Thunb. Fruit Extract

• Published in: Preventive nutrition and food science Vol. 25; no. 4; pp. 380 - 388
• Main Authors: Oh, Joohyun, Han, Yoonyoung, Kim, Jimin, Park, Chansung, Oh, Doolri, Yun, Hyojeong, Lee, Gyuok, Kim, Jaeyong, Choi, Chulyung, Lee, Yongwook
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국식품영양과학회 31-12-2020; The Korean Society of Food Science and Nutrition
• Subjects: 식품과학; Stauntonia hexaphylla (Thunb.) Decaisne; Vaccinium bracteatum Thunb; anti-fatigue activity; exhaustive swimming test; oxidative stress
• ISSN: 2287-1098; 2287-8602
• DOI: 10.3746/pnf.2020.25.4.380

(Thunb.) Decaisne and
Thunb. are commonly used in traditional herbal medicine and food and both exhibit antioxidant and anti-inflammatory effects. Herein, hot-water extracts of
(Thunb.) Decaisne and
Thunb. fruits (1:1 mixture) were used to produce a complex extract NET-1601. The anti-fatigue activity of NET-1601 was evaluated in an
oxidative stress model induced by treating C2C12 myotubes with H
O
. An exhaustive swimming test (EST)
model was established using ICR mice. NET-1601-treated C2C12 myotubes (50, 100, and 200 mg/mL) with H
O
-induced oxidative stress displayed significantly increased cell viability and ATP content, but significantly decreased levels of reactive oxygen species. All NET-1601-treated EST models demonstrated significantly higher maximum swimming rates than control mice. Furthermore, serum lactate, lactate dehydrogenase activity, non-esterified fatty acid, and intramuscular glycogen levels were higher in NET-1601-treated mice than in control mice. In addition, mRNA levels of regulatory factors involved in muscle mitochondrial fatty acid β-oxidation increased upon NET-1601 treatment. Moreover, catalase, superoxide dismutase, glutathione-
-transferase, and liver glutathione content, and antioxidant activity were higher in NET-1601-treated mice than in control mice. Reduced malondialdehyde levels indicated that NET-1601 treatment inhibited exercise-induced lipid peroxidation. Together, these results suggest that NET-1601 retains antioxidant enzyme activity during oxidative stress, simultaneously enhancing both muscle function via glycogen and fatty acid oxidation, thereby exerting a positive effect on recovery from fatigue.