CD95-dependent bystander lysis caused by CD4+ T helper 1 effectors

• Published in: The Journal of immunology (1950) Vol. 157; no. 7; pp. 2961 - 2968
• Main Authors: Wang, R, Rogers, AM, Ratliff, TL, Russell, JH
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01-10-1996
• Subjects: AIDS/HIV; Animals; Antigen-Presenting Cells - immunology; Cell Line, Transformed; Cells, Cultured; Coculture Techniques; Cystitis, Interstitial - immunology; Cystitis, Interstitial - pathology; Cytotoxicity, Immunologic; Fas Ligand Protein; fas Receptor - immunology; Inflammation - immunology; Interferon-gamma - biosynthesis; Interferon-gamma - secretion; L Cells (Cell Line); Lymphocyte Activation; Lymphoma - pathology; Macrophages, Peritoneal - immunology; Membrane Glycoproteins - deficiency; Membrane Glycoproteins - genetics; Membrane Glycoproteins - physiology; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Organ Specificity; Recombinant Proteins - metabolism; Th1 Cells - immunology; Th1 Cells - secretion; Transfection; Tumor Cells, Cultured; Urothelium - cytology; Urothelium - immunology
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.157.7.2961

CD95-dependent lysis of target cells by CD4+ cells raises important questions of specificity and its potential role in inflammation. Much of what we know about CD95L-dependent killing is in fact bystander killing because the T cells have been previously activated by pharmacologic reagents. In an effort to better understand its significance in a more physiologic setting, we have examined bystander lysis by Th1 cells stimulated by Ag and APC in a murine model system. We find that bystander lysis is readily stimulated by M phi APC but not some epithelial lines, even though they themselves are killed in a CD95-dependent process while serving as APC. Bystander activity requires close proximity between the T cell, the APC, and the bystander target because bystanders are not lysed during coculture in Transwells.