Development of tetra-primers ARMS-PCR assay for the detection of A1551G polymorphism in TLR8 gene of riverine buffalo

• Published in: Journal of Applied Animal Research Vol. 40; no. 1; pp. 17 - 19
• Main Authors: Dubey, P.K, Goyal, S, Aggarwal, J, Gahlawat, S.K, Kathiravan, P, Mishra, B.P, Kataria, R.S
• Format: Journal Article
• Language: English
• Published: Taylor & Francis Group 01-01-2012
• Subjects: alleles; buffalo; buffaloes; cost effectiveness; data analysis; DNA; genotype; polymerase chain reaction; polymorphism; single nucleotide polymorphism; tetra-primers ARMS-PCR; Toll-like receptor 8
• ISSN: 0974-1844; 0971-2119; 0974-1844
• DOI: 10.1080/09712119.2011.620247

In this study, a simple PCR-based tetra-primers amplification refractory mutation system (ARMS)-PCR technique has been developed to screen one of the synonymous Single Nucleotide Polymorphism (SNPs) 1551A > G found in buffalo TLR8 gene. The technique was employed to screen 152 genomic DNA samples represented by various riverine and swamp buffaloes. Analysis of data revealed presence of all the three AA, A/G and GG genotypes in Chilika buffalo – whereas only genotype GG was found in all other riverine and swamp buffaloes. The results indicate allele ‘A’ to be Chilika specific and that the technique of tetra-primers ARMS-PCR can be used as a cost-effective, alternate method to differentiate the genotypes.