The genotoxicity and cytotoxicity of tannery effluent in bullfrog (Lithobates catesbeianus)

• Published in: Chemosphere (Oxford) Vol. 183; pp. 491 - 502
• Main Authors: Montalvão, Mateus Flores, de Souza, Joyce Moreira, Guimarães, Abraão Tiago Batista, de Menezes, Ivandilson Pessoa Pinto, Castro, André Luis da Silva, Rodrigues, Aline Sueli de Lima, Malafaia, Guilherme
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-09-2017
• Subjects: Agro-industrial waste; Amphibians; Animals; Aquatic contamination; DNA Damage; Dose-Response Relationship, Drug; Erythroblasts - chemistry; Erythroblasts - drug effects; Erythroblasts - pathology; Erythrocytes - chemistry; Erythrocytes - drug effects; Erythrocytes - pathology; Erythrocytes, Abnormal - chemistry; Erythrocytes, Abnormal - drug effects; Erythrocytes, Abnormal - pathology; Industrial Waste - analysis; Male; Metals, Heavy - analysis; Metals, Heavy - toxicity; Micronuclei, Chromosome-Defective - chemically induced; Micronucleus Tests; Molecular Structure; Mutagens - analysis; Mutagens - toxicity; Rana catesbeiana; Tanning; Time Factors; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - toxicity; Xenobiotics; Agro-industrial waste; Xenobiotics; Aquatic contamination; Amphibians
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2017.05.080

Some of the most polluting activities occur in bovine skin processing. Tannery generates effluents containing high concentrations of heavy metals and organic compounds. The phases composing the leather production process generate a large volume of tannery effluents that are often discarded in aquatic environments without any previous treatment. However, the effect these xenobiotics have on adult representatives belonging to the class Amphibia remains unknown. Thus, the aim of the present study is to assess the geno- and cytotoxic effects of tannery effluent on adult male bullfrogs (Lithobates castesbeianus) exposed to it. Accordingly, the animals were divided into the following groups: negative control (tannery effluent-free water), positive control (cyclophosphamide), and effluent (water added with 5% tannery effluent). The animals were euthanized for blood collection, and erythrocyte analyses were conducted after 35 and 90 days of exposure. The micronuclei (MN) frequency and the frequency of other nuclear abnormalities in each of the animals in the experimental groups were assessed in 2000 erythrocytes. According to the present results, the exposure to tannery effluents increased MN frequency as well as other nuclear abnormalities (i.e., lobed nuclei, binucleated cell, kidney-shaped nuclei, notched nuclei, and apoptotic cell) in the erythrocytes of animals in the effluent group and in the positive control group after 35 and 90 exposure days. Thus, the current study corroborated the hypothesis that the tannery effluent has aneugenic and clastogenic potential in adult male bullfrogs (L. castesbeianus). The present study is the first to report such effect.
•Exposure to tannery effluent has adverse effects on Lithobates catesbeianus.•Tannery effluent increased MN frequency and other nuclear abnormalities.•Genotoxic effects induced by exposure to tannery effluent in bullfrog were evaluated.