In vitro plant regeneration, phenolic compound production and pharmacological activities of Coleonema pulchellum

• Published in: South African journal of botany Vol. 90; pp. 74 - 79
• Main Authors: Baskaran, P., Moyo, M., Van Staden, J.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-01-2014
• Subjects: Antibacterial activity; Antioxidants; In vitro regeneration; Organic elicitors; Phenolic compounds; Antioxidants; Antibacterial activity; Organic elicitors; Phenolic compounds; In vitro regeneration
• ISSN: 0254-6299; 1727-9321
• DOI: 10.1016/j.sajb.2013.10.005

Effects of plant growth regulators (PGRs) and organic elicitors (OEs) on Coleonema pulchellum in vitro micropropagation, secondary product production and pharmacological activities were evaluated. In vitro, ex vitro and parental plants of C. pulchellum were investigated for their potential to produce phenolic and pharmacological compounds. Different morphogenic characteristics of shoots were obtained with PGRs- and OEs-containing media. A higher number of normal shoots were achieved with a low concentration of thidiazuron (TDZ: 4.5μM). Lesser numbers were found with combinations of TDZ (13.6μM)+indole-3-acetic acid (IAA: 2.9μM); haemoglobin (HB: 300mgl−1) or glutamine (GM: 40μM)+benzyladenine (BA: 8.8μM). Shoots were rooted in vitro and successfully acclimatized. Plant growth regulators and OEs had a significant effect on the synthesis and accumulation of phenolic compounds and flavonoids. In particular, casein hydrolysate (CH) as well as a combination of GM and BA induced high levels of total phenolics and flavonoids during in vitro culture. Cytokinins and OEs had a significant effect on DPPH radical scavenging and antibacterial activities of C. pulchellum extracts. Acclimatized C. pulchellum plants can be used as substitute alternative to natural populations.
•In vitro propagation for Coleonema pulchellum is reported for the first time.•More normal and healthy shoots were obtained with 4.5μM TDZ.•In vitro and ex vitro-grown C. pulchellum exhibited high bioactivities.•The protocol can be applied for mass propagation and plant transformation studies.