Genome-scale mRNA transcriptomic insights into the responses of oilseed rape (Brassica napus L.) to varying boron availabilities

• Published in: Plant and soil Vol. 416; no. 1/2; pp. 205 - 225
• Main Authors: Hua, Yingpeng, Feng, Yingna, Zhou, Ting, Xu, Fangsen
• Format: Journal Article
• Language: English
• Published: Cham Springer 01-07-2017; Springer International Publishing; Springer Nature B.V
• Subjects: Anthocyanins; Arabinogalactan; Biomedical and Life Sciences; Boron; Boron (Nutrient); Brassica; Cell walls; Compartmentation; Ecology; Environmental aspects; Enzymes; Gene expression; Gene regulation; Gene sequencing; Genes; Genomes; Homeostasis; Identification methods; Life Sciences; Messenger RNA; Modulation; Molecular modelling; Next-generation sequencing; Observations; Photosynthesis; Photosynthetic pigments; Pigments; Plant genetics; Plant Physiology; Plant Sciences; Plant species; Plant-soil relationships; Plants (botany); Rape plants; Rapeseed; Reactive oxygen species; Regular Article; Soil Science & Conservation; Toxicity; Transcription; China; Transcriptomic response; L; Digital gene expression (DGE); Boron (B) toxicity; Boron (B) deficiency
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-017-3204-2

Backgrounds and aims Boron (B) is a micronutrient essential for plant normal growth and development. Brassica napus L., one of the leading oil-crop species, is extremely susceptible to deficient and excessive B stresses. Previous studies were concentrated only on the responses of plants to a single B stress only. Here, we aim to provide a comprehensive genome-scale mRNA transcriptomic response of Brassica napus to B deficiency and toxicity. Methods The genome-scale mRNA transcriptome was identified and characterized in the leaves and roots of B. napus exposed to B deficiency and toxicity using the digital gene expression (DGE) high-throughput sequencing platforms. Results Under both B deficiency and B toxicity, the biomasses, B contents and photosynthetic pigments of B. napus were significantly reduced whereas the anthocyanins were greatly increased. The DGE profiling revealed a total of 3798 and 3048 genes differentially expressed under B deficiency and toxicity, respectively. Transcriptional down- or upregulation of the B transporter genes, including BnaBOR1s, BnaBOR2, BnaBOR4s and B channel genes, such as BnaNIPs, BnaTIPs and BnaPIPs, may be indispensable for B homeostasis through the molecular modulation of efficient B uptake, transport, distribution and compartmentation. Transcriptional regulation of hydrolytic enzymes and arabinogalactan-protein genes may contribute to the maintenance of cell wall structure and plasma membrane integrity. Transcriptional modulation of antioxidant enzyme genes was likely to balance the reactive oxygen species of plants under B deficiency and toxicity. Conclusions Our results provide comprehensive insights into the mRNA transcriptome of key components involved in the B homeostasis network in B. napus and enrich our understanding of the molecular mechanisms by which plants adapt to deficient and excessive B conditions.