Genetic divergence in Indian populations of M. rosenbergii using microsatellite markers

• Published in: Aquaculture research Vol. 47; no. 2; pp. 472 - 481
• Main Authors: Mohanty, Padmanava, Sahoo, Lakshman, Pillai, Bindu R, Jayasankar, Pallipuram, Das, Paramananda
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Science 01-02-2016; Blackwell Publishing Ltd; Hindawi Limited
• Subjects: alleles; aquaculture; Brackish; conservation programs; genetic distance; genetic diversity; genetic improvement; genetic markers; genetic variation; heterozygosity; M. rosenbergii; Macrobrachium rosenbergii; microsatellite; microsatellite repeats; population structure; riverine population; shrimp; India; INW, Asia; India, Kerala
• ISSN: 1355-557X; 1365-2109
• DOI: 10.1111/are.12508

Macrobrachium rosenbergii, known as the giant freshwater prawn or Malaysian prawn, is the sixth largest aquaculture species in Asia. Knowledge of genetic diversity of M. rosenbergii is important to support management and conservation programmes, which will subsequently help in sustainable production of this economically important species. This study aimed to analyse the genetic diversity and population structure of five M. rosenbergii populations using 11 microsatellite loci. In analysing 240 samples, the number of alleles, observed heterozygosity (HO) and expected heterozygosity (HE) ranged from 3 to 20, from 0.250 to 0.978 and from 0.556 to 0.944 respectively. The five stocks of M. rosenbergii displayed high level of genetic diversity. Both the FST and amova analyses showed that there was significant genetic differentiation among all populations. The UPGMA dendrogram based on Nei's genetic distance matrix revealed that the Narmada and Mahi populations were in one cluster and Mahanadi and Subarnarekha populations in another single major branch, whereas the Kerala population clearly showed a separate cluster. This information on genetic variation will be useful for genetic improvement and conservation of Indian populations of giant freshwater prawn M. rosenbergii.