Continuous solvent production by Clostridium beijerinckii BA101 immobilized by adsorption onto brick

• Published in: World journal of microbiology & biotechnology Vol. 16; no. 4; pp. 377 - 382
• Main Authors: Qureshi, N, Schripsema, J, Lienhardt, J, Blaschek, H.P
• Format: Journal Article
• Language: English
• Published: Dordrecht Kluwer Academic Publishers 01-06-2000; Springer; Springer Nature B.V
• Subjects: acetone; Acid production; adsorption; Bacteria; Biological and medical sciences; Bioreactors; Biotechnology; bricks; butanol; clay; Clostridium beijerinckii; Ethanol; Fermentation; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Microbiology; n-Butyl alcohol; recycling; scanning electron microscopy; Solvents; Packed bed; Solventogenesis; Ethanol; Clostridiales; Clostridium beijerinckii; Organic solvent; Bioreactor; Clostridiaceae; Production; Microorganism culture; Bacteria; Butanol; Entrapped microorganism; Acetone
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1023/a:1008984509404

The performance of a continuous bioreactor containing Clostridium beijerinckii BA101 adsorbed onto clay brick was examined for the fermentation of acetone, butanol, and ethanol (ABE). Dilution rates from 0.3 to 2.5 h⁻¹ were investigated with the highest solvent productivity of 15.8 g l⁻¹ h⁻¹ being obtained at 2.0 h⁻¹. The solvent yield at this dilution rate was found to be 0.38 g g⁻¹ and total solvent concentration was 7.9 g l⁻¹. The solvent yield was maximum at 0.45 at a dilution rate of 0.3 h⁻¹. The maximum solvent productivity obtained was found to be 2.5 times greater than most other immobilized continuous and cell recycle systems previously reported for ABE fermentation. A higher dilution rate (above 2.0 h⁻¹) resulted in acid production rather than solvent production. This reactor was found to be stable for over 550 h. Scanning electron micrographs (SEM) demonstrated that a large amount of C. beijerinckii cells were adsorbed onto the brick support.