Effect of gamma irradiation on the efficacy of β-glucan against acetaminophen induced toxicity in mice

• Published in: Chemico-biological interactions Vol. 180; no. 1; pp. 98 - 105
• Main Authors: Lee, Ju-Woon, Byun, Eui-Hong, Sung, Nak-Yun, Raghavendran, Hanumantha Rao Balaji, Byun, Eui-Baek, Kim, Jae-Hun, Choi, Jong-il, Shin, Myung-Gon, Byun, Myung-Woo
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 15-06-2009
• Subjects: Acetaminophen; Acetaminophen - toxicity; Analgesics, Non-Narcotic - toxicity; Animals; beta-Glucans - pharmacology; beta-Glucans - radiation effects; Centrilobular necrosis; Edema; Gamma irradiation; Gamma Rays; Inflammation; Kidney - cytology; Kidney - drug effects; Liver - cytology; Liver - drug effects; Liver - enzymology; Lung - cytology; Lung - drug effects; Mice; Mice, Inbred BALB C; β-glucan; β-glucan; Centrilobular necrosis; Edema; Gamma irradiation; Inflammation; Acetaminophen
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2008.11.018

The present study was conducted to compare the efficacy of unirradiated β-glucan (UBG) and gamma irradiated β-glucan (GIBG) against acetaminophen (APAP) induced hepatotoxicity in mice. Mice of BALB/c strain were pretreated with UBG and GIBG (50
mg/kg, p.o.) for 7 days and on the 8th day they received an overdose of APAP (500
mg/kg, i.p.). Eight hours after the APAP injection, the levels of serum aminotransferase (AST) and alanine aminotransferase (ALT) were measured and liver, kidney and lung tissue were examined for morphological changes. A significant elevation (
p
<
0.001) of the levels of AST and ALT was observed in mice toxicated with APAP. Histology data revealed severe liver centrilobular necrosis, portal vein damage with apparent toxicity in renal glomerulus and lung inflammation associated with edema. However, a significant inhibition (
p
<
0.05) in the elevation of AST and ALT was observed in mice that received UBG and GIBG compared with APAP-treated mice. Histology examination revealed the non-statistical difference between the protective effects of GIBG and UBG against acetaminophen challenge. In conclusion, it was demonstrated that gamma irradiation induced no severe alteration in the protective activity of β-glucan against APAP-induced hepatotoxicity.