Settlement of the diazotrophic, phytoeffective bacterial strain Pantoea agglomerans on and within winter wheat: an investigation using ELISA and transmission electron microscopy

• Published in: Plant and soil Vol. 145; no. 2; pp. 261 - 273
• Main Authors: Ruppel, S. (Research Centre for Soil Fertility, Muencheberg (Germany)), Hecht-Buchholz, C, Remus, R, Ortmann, U, Schmelzer, R
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-09-1992
• Subjects: Agronomy. Soil science and plant productions; BACTERIA; Biochemistry and biology; Biological and medical sciences; Chemical, physicochemical, biochemical and biological properties; CULTIVOS DE INVIERNO; CULTURE D'HIVER; ELISA; Fundamental and applied biological sciences. Psychology; INOCULACION; INOCULATION; INOCULATION METHODS; Microbiology; MICROSCOPIA; MICROSCOPIE; MICROSCOPY; Pantoea agglomerans; Physics, chemistry, biochemistry and biology of agricultural and forest soils; PLANT RESPONSE; REPONSE DE LA PLANTE; RESPUESTA DE LA PLANTA; Soil science; TEST ELISA; TRITICUM AESTIVUM; WINTER CROPS; Monocotyledones; Internal; Cereal crop; Stem; Immunological method; Microbiological method; Enterobacter agglomerans; Inoculation; Gramineae; Angiospermae; Bacteria; Associated microflora; Plant growth promoting rhizobacteria; Biological treatment; Diazotrophy; Enterobacteriaceae; Laboratory study; Root; Vegetal microorganism relation; Plant leaf; Survival; Transmission electron microscopy; External; Population dynamics; Spermatophyta; ELISA assay; Colonization; Triticum aestivum
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/bf00010355

The aim of this study was to investigate the ability of Pantoea agglomerans , a plant growth-promoting bacterium, to colonize various regions and tissues of the wheat plant (Triticum aestivum L.) by using different inoculation methods and inoculum concentrations. In addition, the enzyme-linked immunosorbent assay (ELISA) and transmission electron microscopy (TEM) were used to determine: (a) the ability of the bacterial cells to grow and survive both on the surface and within internal tissue of the plant and (b) the response of the plant to bacterial infection. After inoculation, cells of the diazotrophic bacterial strain P. agglomerans were found to be located in roots, stems and leaves. Colony development of bacterial cells was only detected within intercellular spaces of the root and on the root surface. However, single bacterial cells were observed in leaves and stems on the surface of the epidermis, in the vicinity to stomatal cells, within intercellular spaces of the mesophyll and within xylem vessels.