Evaluation of the E test for antifungal susceptibility testing of Candida glabrata

• Published in: European journal of clinical microbiology & infectious diseases Vol. 19; no. 2; pp. 146 - 148
• Main Authors: FAVEL, A, CHASTIN, C, THOMET, A. L, REGLI, P, MICHEL-NGUYEN, A, PENAUD, A
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-02-2000; Springer Nature B.V
• Subjects: AE test; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antifungal agents; Antifungal Agents - pharmacology; Biological and medical sciences; Candida - drug effects; Candida glabrata; Candidiasis - microbiology; Culture Media; Evaluation Studies as Topic; Humans; Medical sciences; Microbial Sensitivity Tests - methods; Pharmacology. Drug treatments; Candidiasis; Mycosis; Fluconazole; Drug susceptibility test; Candida glabrata; Method; Fungi; Infection; Antifungal agent; Amphotericin B; Polyenic compound; Minimum inhibitory concentration; Flucytosine; Triazole derivatives; Fungi Imperfecti; Comparative study; Thallophyta; Itraconazole
• ISSN: 0934-9723; 1435-4373
• DOI: 10.1007/s100960050448

The E test was compared to the reference NCCLS broth macrodilution method for susceptibility testing of Candida (Torulopsis) glabrata. The MICs of amphotericin B, flucytosine, fluconazole and itraconazole were determined using the appropriate culture media (RPMI 1640 agar with 2% glucose, Casitone agar or Antibiotic Medium 3 agar) according to the drug tested. Agreement between the two methods was within plus/minus two dilutions for 77-100% of test results, according to the drug/medium combination. The study revealed problems in determining the MICs of azoles using the E test, and confirmed the suitability of Casitone agar for susceptibility testing of fluconazole even if results were read within 24 h.