Contents of Metal(loid)s in a Traditional Ethiopian Flat Bread (Injera), Dietary Intake, and Health Risk Assessment in Addis Ababa, Ethiopia

• Published in: Biological trace element research Vol. 198; no. 2; pp. 732 - 743
• Main Authors: Woldetsadik, Desta, Llorent-Martínez, Eulogio J., Ortega-Barrales, Pilar, Haile, Abinet, Hailu, Hillette, Madani, Nelly, Warner, Noah S., Fleming, David E. B.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-12-2020; Springer Nature B.V
• Subjects: Aluminum; Biochemistry; Biomedical and Life Sciences; Biotechnology; Bread; Carcinogens; Chromium; Daily; Diet; Dietary intake; Flour; Fluorescence; Food; Food intake; Health risk assessment; Health risks; Heavy metals; Inductively coupled plasma mass spectrometry; Iron; Life Sciences; Manganese; Mass spectrometry; Mass spectroscopy; Metals; Nutrition; Oncology; Quotients; Refugees; Risk assessment; Spices; X-ray fluorescence; Ethiopia; Addis Ababa Ethiopia; Addis Ababa; Recommended dietary allowance; Target hazard quotient; Injera; Cancer risk
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-020-02099-7

The traditional Ethiopian flat bread, injera, is a regular component of daily diets in Ethiopia and Eritrea. This bread is also popular among urban refugees particularly Eritreans in Addis Ababa. The levels of metal(loid)s in 40 composite (120 sub-samples) injera samples, representing 4 types of market establishments in Addis Ababa, were determined using inductively coupled plasma-mass spectrometry (ICP-MS) and portable X-ray fluorescence (PXRF). For ICP-MS analysis, the accuracy of the method was evaluated by the analysis of a certified reference material and recovery experiments. It was found that the correlations between the mean levels of Al and Fe and between Al and Mn in injera were highly significant (
p
 < 0.001). It was also found that 1.5 fresh injeras would cover 48–75% of recommended dietary allowance (RDA) for Mg, 17–21% of RDA for K, 19–23% of RDA for Ca, and 60–72% of RDA for P for an adult group aged between 19 and 50. Daily intakes of Al, Fe, and Mn were found to be above the provisional tolerable daily intake (PTDI)/maximum tolerable daily intake (MTDI) values. The mean target hazard quotient (THQ) values for Fe and Mn were greater than 1. The total THQ values varied from 6.52 to 8.53 among market establishments. Estimating carcinogenic risk due to exposure to As, Cr, and Pb indicated that perennial injera consumers might remain at cancer risk. This would further escalate if other staple food items and spices are considered. Hence, there is a need for home-based strategies to reduce extrinsic soil-Al-Fe-Mn in injera/tef batter.