Antioxidant Effect of Hydroxytyrosol, Hydroxytyrosol Acetate and Nitrohydroxytyrosol in a Rat MPP+ Model of Parkinson’s Disease

• Published in: Neurochemical research Vol. 46; no. 11; pp. 2923 - 2935
• Main Authors: Pérez-Barrón, Gabriela, Montes, Sergio, Aguirre-Vidal, Yoshajandith, Santiago, Marti, Gallardo, Elena, Espartero, José Luis, Ríos, Camilo, Monroy-Noyola, Antonio
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-11-2021; Springer Nature B.V
• Subjects: 1-Methyl-4-phenylpyridinium - toxicity; Acetates - administration & dosage; Acetic acid; Administration, Intravenous; Animals; Antioxidants; Antioxidants - administration & dosage; Biochemistry; Biomedical and Life Sciences; Biomedicine; Catechols - administration & dosage; Cell Biology; Corpus Striatum - drug effects; Corpus Striatum - metabolism; Dopamine; Dopamine - metabolism; Ethanol; Fluorescence; Lipid Peroxidation - drug effects; Lipid Peroxidation - physiology; Lipids; Male; Markers; Movement disorders; MPP; Neostriatum; Neurochemistry; Neurodegenerative diseases; Neurology; Neurosciences; Original Paper; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - physiology; Parkinson's disease; Parkinsonian Disorders - chemically induced; Parkinsonian Disorders - metabolism; Parkinsonian Disorders - prevention & control; Phenylethyl Alcohol - administration & dosage; Phenylethyl Alcohol - analogs & derivatives; Preservation; Rats; Rats, Wistar; Saline solutions; Treatment Outcome; Hydroxytyrosol; Lipid peroxidation; Oxidative stress; Dopamine; Parkinson’s disease; GSH/GSSG
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-021-03379-x

3,4-Dihydroxyphenyl ethanol, known as hydroxytyrosol (HTy), is a phenylpropanoid found in diverse vegetable species. Several studies have demonstrated that HTy is a potent antioxidant. Thus, our study is aimed to evaluate the antioxidant effect of HTy and its derivatives, hydroxytyrosol acetate (HTyA) and nitrohydroxytyrosol (HTyN), in a model of oxidative stress induced by 1-methyl-4-phenylpyridinium (MPP
+
) in rats. Rats were administered intravenously (i.v.) in the tail with 1 mL saline solution or polyphenol compound (1.5 mg/kg) 5 min before intrastriatal infusion of 10 µg MPP
+
/8 µL. We found that rats injured with MPP
+
, pretreatment with HTy, HTyA or HTyN significantly decreased ipsilateral turns. This result was consistent with a significant preservation of striatal dopamine levels and decreased lipid fluorescence products (LFP), a marker of oxidative stress. Brain GSH/GSSG ratio, from rats pretreated with HTy or HTyN showed a significant preservation of that marker, decreased as a consequence of MPP
+
-induced oxidative damage. These results show an antioxidant effect of HTy, HTyA and HTyN in the MPP
+
model of Parkinson’s disease in the rat.