Tissue sampling is non-inferior in comparison to sonication in orthopedic revision surgery

• Published in: Archives of orthopaedic and trauma surgery Vol. 143; no. 6; pp. 2901 - 2911
• Main Authors: Fritsche, Theresa, Schnetz, Matthias, Klug, Alexander, Fischer, Sebastian, Ruckes, Christian, Hunfeld, K. P., Hoffmann, Reinhard, Gramlich, Yves
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-06-2023; Springer Nature B.V
• Subjects: Antibiotics; Arthritis, Infectious - diagnosis; Humans; Infections; Medicine; Medicine & Public Health; Orthopaedic Surgery; Orthopedics; Prostheses and Implants; Prosthesis-Related Infections - diagnosis; Prosthesis-Related Infections - microbiology; Reoperation; Sensitivity and Specificity; Sonication - methods; Sonication; Sonification; Periprosthetic joint infection; Tissue samples; PJI; Musculoskeletal infection
• ISSN: 1434-3916; 0936-8051; 1434-3916
• DOI: 10.1007/s00402-022-04469-3

Background
The aim of this study was to assess the role of sonication fluid cultures in detecting musculoskeletal infections in orthopedic revision surgery in patients suspected of having peri-prosthetic joint infection (PJI), fracture-related infections (FRI), or postoperative spinal implant infections (PSII).
Methods
Between 2016 and 2019, 149 cases with a data set including sonication fluid cultures and tissue specimen and histological analysis were included. Accuracy of each diagnostic tool as well as the influence of antibiotic therapy was analyzed. Pathogens identified in the sonication cultures and in the associated tissue samples were compared based on the matching of the antibiograms. Therapeutic benefits were then assessed.
Results
Of 149 cases, 43.6% (
n
 = 65) were identified as PJI, 2.7% (
n
 = 4) as FRI, 12.8% (
n
 = 19) as PSII, 6.7% (
n
 = 10) as aseptic non-union, and 34.2% (
n
 = 51) as aseptic implant loosening. The sensitivity and specificity of tissue and synovial specimens showed no significant difference with respect to sonication fluid cultures (sensitivity/specificity: tissue: 68.2%/96.7%; sonication fluid cultures: 60.2%/98.4%). The administration of antibiotics over 14 days prior to microbiological sampling (
n
 = 40) resulted in a lower sensitivity of 42.9% each. Histological analysis showed a sensitivity 86.3% and specificity of 97.4%. In 83.9% (
n
 = 125) of the cases, the results of sonication fluid cultures and tissue specimens were identical. Different microorganisms were found in only four cases. In 17 cases, tissue samples (
n
 = 5) or sonication (
n
 = 12) were false-negatives.
Conclusion
Sonication fluid culture showed no additional benefit compared to conventional microbiological diagnostics of tissue and synovial fluid cultures. Preoperative administration of antibiotics had a clearly negative effect on microbiologic test accuracy. In over 83.9% of the cases, sonication fluid and tissue cultures showed identical results. In the other cases, sonication fluid culture did not further contribute to the therapy decision, whereas other factors, such as fistulas, cell counts, or histological analysis, were decisive in determining therapy.