Disease-associated astrocyte epigenetic memory promotes CNS pathology

• Published in: Nature (London) Vol. 627; no. 8005; pp. 865 - 872
• Main Authors: Lee, Hong-Gyun, Rone, Joseph M., Li, Zhaorong, Akl, Camilo Faust, Shin, Seung Won, Lee, Joon-Hyuk, Flausino, Lucas E., Pernin, Florian, Chao, Chun-Cheih, Kleemann, Kilian L., Srun, Lena, Illouz, Tomer, Giovannoni, Federico, Charabati, Marc, Sanmarco, Liliana M., Kenison, Jessica E., Piester, Gavin, Zandee, Stephanie E. J., Antel, Jack P., Rothhammer, Veit, Wheeler, Michael A., Prat, Alexandre, Clark, Iain C., Quintana, Francisco J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28-03-2024; Nature Publishing Group
• Subjects: 45/15; 45/23; 45/44; 45/91; 631/250/371; 631/378/1689; Accessibility; Acetyl Coenzyme A - metabolism; Animals; Astrocytes; Astrocytes - enzymology; Astrocytes - metabolism; Astrocytes - pathology; ATP Citrate (pro-S)-Lyase - metabolism; Autoimmune diseases; Chromatin; Chromatin - genetics; Chromatin - metabolism; Chromatin Assembly and Disassembly; Chromatin Immunoprecipitation Sequencing; Coenzyme A; CRISPR; CRISPR-Cas Systems; Cytokines; Disease; Encephalomyelitis, Autoimmune, Experimental - enzymology; Encephalomyelitis, Autoimmune, Experimental - genetics; Encephalomyelitis, Autoimmune, Experimental - metabolism; Encephalomyelitis, Autoimmune, Experimental - pathology; Epigenetic Memory; Epigenetics; Experimental allergic encephalomyelitis; Female; Gene expression; Gene sequencing; Histone acetyltransferase; Humanities and Social Sciences; Humans; Immunohistochemistry; Immunoprecipitation; In vivo methods and tests; Inactivation; Inflammation; Inflammation - enzymology; Inflammation - genetics; Inflammation - metabolism; Inflammation - pathology; Interrogation; Lymphocytes; Male; Metabolism; Mice; multidisciplinary; Multiple sclerosis; Multiple Sclerosis - enzymology; Multiple Sclerosis - genetics; Multiple Sclerosis - metabolism; Multiple Sclerosis - pathology; Neurological diseases; Nucleic acids; Pathology; Phenotypes; Ribonucleic acid; RNA; Science; Science (multidisciplinary); Single-Cell Gene Expression Analysis; Transposase; Transposases - metabolism
• ISSN: 0028-0836; 1476-4687; 1476-4687
• DOI: 10.1038/s41586-024-07187-5

Disease-associated astrocyte subsets contribute to the pathology of neurologic diseases, including multiple sclerosis and experimental autoimmune encephalomyelitis
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(EAE), an experimental model for multiple sclerosis. However, little is known about the stability of these astrocyte subsets and their ability to integrate past stimulation events. Here we report the identification of an epigenetically controlled memory astrocyte subset that exhibits exacerbated pro-inflammatory responses upon rechallenge. Specifically, using a combination of single-cell RNA sequencing, assay for transposase-accessible chromatin with sequencing, chromatin immunoprecipitation with sequencing, focused interrogation of cells by nucleic acid detection and sequencing, and cell-specific in vivo CRISPR–Cas9-based genetic perturbation studies we established that astrocyte memory is controlled by the metabolic enzyme ATP-citrate lyase (ACLY), which produces acetyl coenzyme A (acetyl-CoA) that is used by histone acetyltransferase p300 to control chromatin accessibility. The number of ACLY
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p300
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memory astrocytes is increased in acute and chronic EAE models, and their genetic inactivation ameliorated EAE. We also detected the pro-inflammatory memory phenotype in human astrocytes in vitro; single-cell RNA sequencing and immunohistochemistry studies detected increased numbers of ACLY
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p300
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astrocytes in chronic multiple sclerosis lesions. In summary, these studies define an epigenetically controlled memory astrocyte subset that promotes CNS pathology in EAE and, potentially, multiple sclerosis. These findings may guide novel therapeutic approaches for multiple sclerosis and other neurologic diseases.
In an experimental autoimmune encephalomyelitis model in mice, a subset of astrocytes retains an epigenetically regulated memory of past inflammation, causing exacerbated inflammation upon subsequent rechallenge.