Polymorphism rs1800255 from COL3A1 gene and the risk for pelvic organ prolapse

• Published in: International Urogynecology Journal Vol. 31; no. 1; pp. 73 - 78
• Main Authors: Teixeira, Fernando Henrique, Fernandes, César Eduardo, do Souto, Ricardo Peres, de Oliveira, Emerson
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 2020; Springer Nature B.V
• Subjects: Aged; Case-Control Studies; Childbirth & labor; Collagen; Collagen Type III - genetics; Female; Genes; Genetic Predisposition to Disease; Gynecology; Health risk assessment; Humans; Medicine; Medicine & Public Health; Middle Aged; Original Article; Pelvic organ prolapse; Pelvic Organ Prolapse - genetics; Polymorphism; Polymorphism, Single Nucleotide; Studies; Urology; Womens health; Pelvic organ prolapse; Single-nucleotide polymorphism; Restriction fragment length polymorphism; Type III collagen
• ISSN: 0937-3462; 1433-3023
• DOI: 10.1007/s00192-019-03965-2

Introduction and hypothesis
Genetic variations of type III collagen may compromise the supportive structures of the female pelvic floor and consequently favor pelvic organ prolapse. The single nucleotide polymorphism G/A rs1800255 located in the coding region for type III collagen (
COL3A1
) was evaluated as a risk factor for pelvic organ prolapse.
Methods
A single-center prospective cohort study including women with clinical diagnosis of stage III and IV prolapse (POP group) and prolapse stage 0 or I (control group). Sociodemographic, clinical data and obstetric history were retrieved by physician interview. DNA including the rs1800255 polymorphism was amplified by polymerase chain reaction from blood genomic cells and digested with
Alu
I restriction enzyme for distinction of G and A variants. Qualitative variables were compared using the chi-square and Fisher’s exact tests and unpaired
t
-test for quantitative variables. After stratification of the groups, risk factors for POP were estimated using odds ratios (ORs) from the binary logistic regression model.
Results
A total of 292 women were included, 112 in the POP group and 180 in the control group. There was no significant difference between groups regarding rs1800255. Age and home birth were the only significant risk factors for pelvic organ prolapse.
Conclusion
Polymorphism rs1800255 from
COL3A1
gene was not a risk factor for pelvic organ prolapse.