Accessions of Brazilian ginseng (Pfaffia glomerata) with contrasting anthocyanin content behave differently in growth, antioxidative defense, and 20-hydroxyecdysone levels under UV-B radiation

• Published in: Protoplasma Vol. 256; no. 6; pp. 1557 - 1571
• Main Authors: Felipe, Sérgio Heitor Sousa, Batista, Diego Silva, Chagas, Kristhiano, Correia, Ludmila Nayara Freitas, Silva, Tatiane Dulcineia, Fortini, Evandro Alexandre, Silva, Priscila Oliveira, Otoni, Wagner Campos
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-11-2019; Springer Nature B.V
• Subjects: Agrochemicals; Anthocyanins - metabolism; Biomedical and Life Sciences; Brazil; Cell Biology; Electron transport; Gene expression; Ginseng; Life Sciences; Metabolites; Original Article; Panax - chemistry; Pfaffia; Plant Sciences; Secondary metabolites; Stress, Physiological; Sucrose; Tissue culture; Ultraviolet radiation; Ultraviolet Rays; Zoology; Brazil; Physical elicitor; Amaranthaceae; Ultraviolet-B; Abiotic stress; Phytoecdysteroid
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-019-01400-3

Ultraviolet-B (UV-B) radiation is an elicitor of secondary metabolites in plant tissue culture, but the effects on 20-hydroxyecdysone (20E) are still unclear. The 20E may show biotechnological, pharmacological, medical, and agrochemical applicability. Here, we use
Pfaffia glomerata
, a medically important species, to understand the impacts of UV-B radiation on their physiological performance, the expression of key genes involved in the 20E biosynthesis, and the 20E content. Two accessions (A22 and A43) of plants 20 days old grown in vitro were exposed to 0 (control), 2 (6.84 kJ m
−2
), and 4 (13.84 kJ m
−2
) h UV-B radiation for 20 consecutive days. Our data showed that UV-B reduced glucose concentration in A22 and A43 under 4 h of exposure (29 and 30%, respectively), while sucrose concentration increased (32 and 57%, respectively). UV-B also differentially impacted the accessions (A22 and A43), where the A22 under 4 h of UV-B had reduced total dry weight (8%) and electron transport rate (31%); in contrast, A43 did not change. Also, only A22 had increased POD activity under 4 h of UV-B (66%), as well as increased gene expression of the 20E pathway and the 20E content under 2 and 4 h of UV-B in leaves (28 and 21%, respectively) and roots (16 and 13%, respectively). This differential performance to UV-B can be explained by the contrasting anthocyanin contents. Notably, A43 displayed 56% more anthocyanin to the former, a possible defense against UV-B. In conclusion, UV-B radiation is a potential elicitor for increasing 20E content in
P. glomerata
grown in vitro.