Sustained Release of Green Tea Polyphenols from Liposomal Nanoparticles; Release Kinetics and Mathematical Modelling

• Published in: Iranian journal of biotechnology Vol. 15; no. 4; pp. 277 - 283
• Main Authors: Prakash Upputuri, Ravi Theaj, Azad Mandal, Abul Kalam
• Format: Journal Article
• Language: English
• Published: National Institute of Genetic Engineering and Biotechnology 29-12-2017
• ISSN: 1728-3043; 2322-2921
• DOI: 10.15171/ijb.1322

Background:
Green tea polyphenols (GTP) are known to have several health benefits. In spite of these benefits, its application
as a therapeutic agent is limited due to some of its limitations such as stability, bioavailability, and biotransformation. To
overcome these limitations, liposomal nanoparticles have been used as a carrier of the GTP.
Objective:
Encapsulation of GTP to the liposomal nanoparticles in order to achieve a sustained release of the GTP and to
determine the drug release kinetics and the mechanism of the release.
Materials and Methods:
GTP encapsulated liposomal nanoparticles were prepared using phosphatidyl choline and
cholesterol. The synthesized particles were characterized for their particle size and morphology.
In vitro
release studies
were carried out, followed by drug release kinetics, and determining the mechanism of release.
In vitro
, antioxidant assay
was determined following 2,2-diphenyl-1-picrylhydrazyl (DPPH) method.
Results:
Atomic force microscope (AFM) and high resolution scanning electron microscope (HR SEM) images showed
spherical particles of the size of 64.5 and 252 nm. An encapsulation efficiency as high as 77.7% was observed with GTP
concentration of 5 mg.mL
-1
.
In vitro
release studies showed that the loading concentrations of GTP were independent to
the cumulative percentage of the drug release. GTP release by varying the pH and temperature showed a direct correlation
between the release parameter and the percentage of drug release. The higher the pH and temperature, the higher was the
percentage of the drug release. The release data showed a good correlation with Zero order kinetics and the mechanism of
the release being anomalous mode. Radical scavenging activity of the released GTP showed a potent scavenging activity.
Conclusion:
GTP encapsulated liposomal nanoparticles could be used as a delivery vehicle for achieving a sustained release.