Molecular characterization and hematological analysis of Listeria monocytogenes infection in dairy cows in Punjab (Pakistan)

• Published in: Archives of microbiology Vol. 204; no. 4; p. 201
• Main Authors: Munir, Muhammad Zubair, Khan, Jawaria Ali, Ijaz, Muhammad, Akhtar, Fareeha
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-04-2022; Springer Nature B.V
• Subjects: Animal diseases; Animals; Bacterial diseases; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cattle; Cell Biology; Chi-square test; Cow's milk; Dairy cattle; Ecology; Female; Food irradiation; Food safety; hlyA gene; Humans; Leukocytes; Leukocytes (neutrophilic); Life Sciences; Listeria; Listeria monocytogenes; Listeria monocytogenes - genetics; Listeriosis; Listeriosis - epidemiology; Listeriosis - microbiology; Listeriosis - veterinary; Microbial Ecology; Microbiology; Milk; Milk - microbiology; Original Paper; Pakistan; Phylogeny; Polymerase chain reaction; Statistical tests; Pakistan; Punjab Pakistan; Phylogenetic analysis; Cows; Pakistan; Listeria monocytogenes
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-022-02804-1

Listeriosis is an emerging bacterial disease of animals and humans worldwide, caused by
Listeria monocytogenes
. The infected dairy cows continuously shed the microbes in their milk, a human being’s concern. This study was designed to molecular characterize the
Listeria monocytogenes
isolated from symptomatic cow's milk of tehsils Samundri, Gujar khan, and Alipur of Punjab. A total of 175 milk samples were collected, pre-enriched and cultured on PALCAM agar. The affirmation of the
hly
A gene of
Listeria monocytogenes
was performed by polymerase chain reaction (PCR) and 3.43% of isolates were found positive. The phylogenetic analysis showed a resemblance of our isolates of
Listeria monocytogenes
with India (KP965733), the USA (DQ812484), and 3 of our isolates made a clade. The leucocytes and neutrophils count were found significantly increased in listeriosis affected cows. The Chi-square test showed a significant association between poor quality silage feeding and listeriosis. The presence of
L.monocytogenes
in cow’s milk indicates a potential threat to humans. It is further recommended that it should be consistently monitored to ensure food safety.