LC–MS/MS-based profiling of bioactive metabolites of endophytic bacteria from Cannabis sativa and their anti-Phytophthora activity

• Published in: Antonie van Leeuwenhoek Vol. 114; no. 8; pp. 1165 - 1179
• Main Authors: Iqrar, Irum, Numan, Muhammad, Khan, Tariq, Shinwari, Zabta Khan, Ali, Gul Shad
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-08-2021; Springer Nature B.V
• Subjects: Abnormalities; Acetic acid; Antiinfectives and antibacterials; Bacteria; Bioactive compounds; Biological activity; Biological control; Biomedical and Life Sciences; Cannabis; Cannabis sativa; Cotinine; Crops; Disease control; Endophytes; Enterobacter cloacae; Ethyl acetate; High-performance liquid chromatography; Hyphae; Lesions; Levodopa; Life Sciences; Liquid chromatography; Lysine; Medical Microbiology; Metabolites; Microbiology; Original Paper; Ornithine; Paenibacillus; Pesticides; Phytophthora; Plant protection; Plant Sciences; Rhizosphere; Soil Science & Conservation; Tobacco; Tryptophan; Medicinal plants; Microbial bioactive compounds; Endophytic bacteria; Biocontrol agents; Cannabis sativa; Phytophthora parasitica
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-021-01586-8

Protection of crop plants from phytopathogens through endophytic bacteria is a newly emerged area of biocontrol. In this study, endophytic bacteria were isolated from the rhizosphere of
Cannabis sativa
. Based on initial antimicrobial screening, three (03) bacteria
Serratia marcescens
MOSEL-w2,
Enterobacter cloacae
MOSEL-w7, and
Paenibacillus
MOSEL-w13 were selected
.
Antimicrobial assays of these selected bacteria against
Phytophthora parasitica
revealed that
E. cloacae
MOSEL-w7
and Paenibacillus
sp. MOSEL-w13 possessed strong activity against
P. parasitica.
All these bacterial extracts showed strong inhibition against
P. parasitica
at different concentrations (4–400 µg mL
−1
).
P. parasitica
hyphae treated with ethyl acetate extract of
E. cloacae
MOSEL-w7 resulted in severe growth abnormalities compared to control. The extracts were further evaluated for in vivo detached-leaf assay against
P. parasitica
on the wild type tobacco. Application of 1% ethyl acetate bacterial extract of
S. marcescens
MOSEL-w2,
E. cloacae
MOSEL-w7, and
Paenibacillus
sp. MOSEL-w13 reduced
P. parasitica
induced lesion sizes and lesion frequencies by 60–80%. HPLC based fractions of each extract also showed bioactivity against
P. parasitica
. A total of 24 compounds were found in the
S. marcescens
MOSEL-w2, 15 compounds in
E. cloacae
MOSEL-w7 and 20 compounds found in
Paenibacillus
sp. MOSEL-w13. LC–MS/MS analyses showed different bioactive compounds in the bacterial extracts such as Cotinine (alkylpyrrolidine),
l
-tryptophan,
l
-lysine,
l
-Dopa, and
l
-ornithine. These results suggest that
S. marcescens
MOSEL-w2,
E. cloacae
MOSEL-w7, and
Paenibacillus
MOSEL-w13 are a source of bioactive metabolites and could be used in combination with other biocontrol agents, with other modes of action for controlling diseases caused by
Phytophthora
in crops. They could be a clue for the broad-spectrum biopesticides for agriculturally significant crops.