Impact of Bacillus cereus SPB-10 on Growth Promotion of Wheat (Triticum aestivum L.) Under Arsenic-Contaminated Soil

• Published in: Current microbiology Vol. 81; no. 6; p. 153
• Main Authors: Chauhan, Prabhat K., Pathak, Himanshu K., Dubey, Gopal, Sharma, Hritik, Upadhyay, Sudhir K.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-06-2024; Springer Nature B.V
• Subjects: Arsenic; Arsenic - metabolism; Arsenic ions; Bacillus cereus; Bacillus cereus - drug effects; Bacillus cereus - genetics; Bacillus cereus - growth & development; Bacillus cereus - metabolism; Bacteria; Biodegradation, Environmental; Biomedical and Life Sciences; Biotechnology; Chlorophyll; Essential nutrients; Life Sciences; Microbiology; Nutrients; Plant growth; Plant roots; Plant Roots - growth & development; Plant Roots - microbiology; Polysaccharides; RNA, Ribosomal, 16S - genetics; rRNA 16S; Siderophores; Siderophores - metabolism; Soil contamination; Soil investigations; Soil Microbiology; Soil Pollutants - metabolism; Soil pollution; Soils; Solubilization; Triticum - growth & development; Triticum - metabolism; Triticum - microbiology; Triticum aestivum; Wheat
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-024-03673-1

This study investigates the impact of bacteria on arsenic reduction in wheat plants, highlighting the potential of microbe-based eco-friendly strategies for plant growth. In the present study, bacterial isolate SPB-10 was survived at high concentration against both form of arsenic (As
3+
and As
5+
). SPB-10 produced 5.2 g/L and 11.3 g/L of exo-polysaccharide at 20 ppm of As
3+
and As
5+
, respectively, whereas qualitative examination revealed the highest siderophores ability. Other PGP attributes such as IAA production were recorded 52.12 mg/L and 95.82 mg/L, phosphate solubilization was 90.23 mg/L and 129 mg/L at 20 ppm of As
3+
and As
5+
, respectively. Significant amount of CAT, APX, and Proline was also observed at 20 ppm of As
3+
and As
5+
in SPB-10. Isolate SPB-10 was molecularly identified as
Bacillus cereus
through 16S rRNA sequencing. After 42 days, wheat plants inoculated with SPB-10 had a 25% increase in shoot length and dry weight, and 26% rise in chlorophyll-a pigment under As
5+
supplemented T4 treatment than control. Reducing sugar content was increased by 24% in T6-treated plants compared to control. Additionally, SPB-10 enhanced the content of essential nutrients (NPK), CAT, and APX in plant’s-leaf under both As
3+
and As
5+
stressed conditions after 42 days. The study found that arsenic uptake in plant roots and shoots decreased in SPB-10-inoculated plants, with the maximum reduction observed in As
5+
treated plants. Bio-concentration factor-BCF was reduced by 90.89% in SPB-10-inoculated treatment T4 after 42 days. This suggests that
Bacillus cereus
-SPB-10 may be beneficial for plant growth in arsenic-contaminated soil.