Dynamic distribution of ARGONAUTE1 (AGO1) and ARGONAUTE4 (AGO4) in Hyacinthus orientalis L. pollen grains and pollen tubes growing in vitro

• Published in: Protoplasma Vol. 257; no. 3; pp. 793 - 805
• Main Authors: Niedojadło, Katarzyna, Kupiecka, Małgorzata, Kołowerzo-Lubnau, Agnieszka, Lenartowski, Robert, Niedojadło, Janusz, Bednarska-Kozakiewicz, Elżbieta
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-05-2020; Springer Nature B.V
• Subjects: Arabidopsis Proteins - chemistry; Argonaute Proteins - chemistry; Biomedical and Life Sciences; Cell Biology; Chromatin; DNA methylation; Gene expression; Gene silencing; Hyacinthus - growth & development; Hyacinthus orientalis; Life Sciences; Localization; Maturation; Original Article; Plant Sciences; Pollen; Pollen - growth & development; Pollen Tube - growth & development; Pollen tubes; Post-transcription; RNA-induced silencing complex; RNA-mediated interference; siRNA; Spatial distribution; Temporal distribution; Zoology; miRNA; Male germ unit (MGU); siRNA; AGO4; Pollen tube; AGO1
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-019-01463-2

The transcriptional and posttranscriptional AGO-mediated control of gene expression may play important roles during male monocot gametophyte development. In this report, we demonstrated dynamic changes in the spatiotemporal distribution of AGO1 and AGO4, which are key proteins of the RNA-induced silencing complex (RISC) in
Hyacinthus orientalis
male gametophyte development. During maturation of the bicellular pollen grains and in vitro pollen tube growth, the pattern of AGO1 localization was correlated with previously observed transcriptional activity of the cells. During the period of high transcriptional activity, AGO1 is associated with chromatin while the clustered distribution of AGO1 in the interchromatin areas is accompanied by condensation of chromatin and the gradual transcriptional silencing of both cells in mature, dehydrated pollen. During pollen tube growth and the restarting of RNA synthesis in the vegetative nucleus, AGO1 is dispersed in the chromatin. Additionally, the gradual increase in the cytoplasmic pool of AGO1 in the elongating pollen tube indicates the activation of the posttranscriptional gene silencing (PTGS) pathway. During pollen tube growth in the generative cell and in the sperm cells, AGO1 is present mainly in the areas between highly condensed chromatin clusters. Changes in the distribution of AGO4 that indicated the possibility of spatiotemporal organization in the RNA-directed DNA methylation (RdDM) process (cytoplasmic and nuclear steps) were also observed during hyacinth male gametophyte development. Based on our findings, we propose that in the germinating pollen tube, the cytoplasmic assembly of AGO4/siRNA takes place and that the mature complexes could be transported to the nucleus to carry out their function during the next steps of pollen tube growth.