Appraisal of different levels of soybean meal in diets on growth, digestive enzyme activity, antioxidation, and gut histology of tilapia (Oreochromis niloticus)

• Published in: Fish physiology and biochemistry Vol. 46; no. 4; pp. 1397 - 1407
• Main Authors: Pervin, Mst. Arzu, Jahan, Halima, Akter, Rabeya, Omri, Abdelwahab, Hossain, Zakir
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-08-2020; Springer Nature B.V
• Subjects: Amylases - analysis; Animal Anatomy; Animal Biochemistry; Animal Feed; Animal Physiology; Animals; Antioxidants; Biomedical and Life Sciences; Body weight; Body weight gain; Caseins - metabolism; Catalase; Catalase - metabolism; Cichlids - anatomy & histology; Cichlids - growth & development; Cichlids - metabolism; Cichlids - physiology; Diet; Digestive enzymes; Digestive system; Enzymatic activity; Enzyme activity; Enzymes; Fish; Fish meal; Fishmeal; Freshwater & Marine Ecology; Freshwater fishes; Gastrointestinal tract; Glutathione Peroxidase - metabolism; Glycine max; Growth rate; Height; Histology; Intestine; Intestines; Intestines - anatomy & histology; Intestines - enzymology; Life Sciences; Lipase; Lipase - metabolism; Liver - enzymology; Marine fishes; Morphology; Oreochromis niloticus; Ponds; Random Allocation; Satiety; Soybeans; Stomach; Stomach - anatomy & histology; Stomach - enzymology; Superoxide dismutase; Superoxide Dismutase - metabolism; Thiobarbituric Acid Reactive Substances - analysis; Tilapia; Villus; Weight Gain; Zoology; Fishmeal; Gut histology; Antioxidation; Digestive enzyme activity; Soybean meal
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-020-00798-5

This study was designed to determine the effect of fishmeal (FM) replacement with soybean meal (SBM) in the diet of tilapia (
Oreochromis niloticus
). Growth, digestive enzyme activity, antioxidation, and gut histomorphology were assessed in the fish fed with five different formulated diets that increasingly replaced FM. The SBM0 contained 100% FM, followed by 25% (SBM25), 50% (SBM50), 75% (SBM75), and 100% (SBM100). Juvenile tilapia having weight and length of 6.6 ± 0.1 g and 5.4 ± 0.2 cm, respectively, were randomly divided into five treatment groups. Each group had 40 individual fish and fed to visual satiation for 90 days. Body weight gain and specific growth rate in fish fed with 25–75% SBM increased significantly (
P
 < 0.01) compared with those in fish fed with SBM100. Fish having the same weight fed with diets SBM50, SBM75, and SBM100 showed a significantly (
P
 < 0.01) longer intestine compared with those fed with SBM0 and SBM25. Villus height of the stomach and intestine was significantly (
P
 < 0.01) greater in the fish fed with the diets SBM0, SBM25, and SBM50 compared with SBM75 and SBM100. Muscular thickness was inversely related with the increasing villus height. Protease activity increased significantly (
P
 < 0.01) in the stomach, anterior intestine, and posterior intestine of fish fed with SBM0 compared with SBM100. In the stomach and anterior and posterior segments of the intestine, significantly (
P
 < 0.01) higher lipase activity was observed in fish fed with the diets SBM0 and SBM25 compared with diet SBM100. In the stomach, anterior intestine, and posterior intestine, amylase activity was also significantly (
P
 < 0.01) greater in SBM0 compared with SBM100. The antioxidant enzymes including superoxide dismutase and catalase of the liver were significantly (
P
 < 0.01) higher in fish fed SBM100 compared with SBM0. These results suggest that the replacement of FM up to 75% with SBM could be possible considering the growth performances, gut health, and activities of digestive enzymes and antioxidant enzymes in
O. niloticus
.