Characterization of Iturin V, a Novel Antimicrobial Lipopeptide from a Potential Probiotic Strain Lactobacillus sp. M31

• Published in: Probiotics and antimicrobial proteins Vol. 13; no. 6; pp. 1766 - 1779
• Main Authors: Singh, Shelley Sardul, Akhtar, Md. Naushad, Sharma, Deepika, Mandal, Santi M., Korpole, Suresh
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-12-2021; Springer Nature B.V
• Subjects: Amino acid composition; Animals; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Applied Microbiology; Biosurfactants; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Cytotoxicity; Dendritic cells; Food processing; Immunomodulation; Inflammation; Interleukin 12; Ionization; Ions; Lactic acid; Lactic acid bacteria; Lactobacillus; Lipopeptides; Lipopeptides - pharmacology; Mice; Microbiology; Molecular weight; Nutrition; Peptides, Cyclic - pharmacology; Probiotics; Protein Science; Tandem Mass Spectrometry; Tumor necrosis factor; Fish; Pro-inflammatory; Iturin V; Lipopeptide; Pseudomonas; Lactobacillus; Vibrio
• ISSN: 1867-1306; 1867-1314
• DOI: 10.1007/s12602-021-09796-2

Members of lactic acid bacteria group are known to produce various antimicrobial substances. Cyclic lipopeptides are one such potent class of amphipathic natural biosurfactants that exhibit bactericidal and immunomodulatory properties. In this study, we aimed to investigate antimicrobial and immunomodulatory activities of a lipopeptide secreted by a LAB isolate strain M31 identified as a member of the genus
Lactobacillus
. The lipopeptide that was purified using a combination of chromatographic techniques and matrix-assisted laser desorption/ionization-time of flight of pure lipopeptide displayed a molecular weight of 1002 Da. MS/MS analysis confirmed the presence of 7 amino acids (Asp-Tyr-Asp-Val-Pro-Asp-Ser) and a C13 beta-hydroxy fatty acid. The amino acid composition assigned lipopeptide to iturin class. However, the replacement of Gln with Val revealed it to represent a novel iturin named as iturin V. Iturin V showed antibacterial activity and did not cause hemolysis or cytotoxicity upto 125 µg/mL. It induced secretion of pro-inflammatory cytokines TNF-alpha and IL-12 in murine dendritic cells. Probiotic features of strain M31 coupled with notable activity of iturin V against species of the genera
Pseudomonas
and
Vibrio
suggest that strain M31 has potential application for pathogen intervention treatments in processing of aquatic food products.