Characterization of Exopolysaccharides (EPSs) Obtained from Ligilactobacillus salivarius Strains and Investigation at the Prebiotic Potential as an Alternative to Plant Prebiotics at Poultry

• Published in: Probiotics and antimicrobial proteins Vol. 14; no. 1; pp. 49 - 59
• Main Authors: Bikric, Seda, Aslim, Belma, Dincer, İlknur, Yuksekdag, Zehranur, Ulusoy, Seyhan, Yavuz, Serkan
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-02-2022; Springer Nature B.V
• Subjects: Adenocarcinoma; Animals; Applied Microbiology; Biofilms; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chromatography; Exopolysaccharides; High-performance liquid chromatography; Intestinal microflora; Lactobacillus - metabolism; Lactobacillus salivarius; Microbiology; Microbiota; Molecular weight; Monomers; NMR; Nuclear magnetic resonance; Nutrition; Polysaccharides, Bacterial - chemistry; Poultry; Prebiotics; Protein Science; Quorum sensing; Anti-biofilm; growth stimulator; Prebiotic; Adhesion; Anti-quorum sensing; Lactobacilli growth stimulator; Ligilactobacillus salivarius
• ISSN: 1867-1306; 1867-1314
• DOI: 10.1007/s12602-021-09790-8

In this study, it was aimed to reveal the potential of using exopolysaccharides (EPS) obtained from
Ligilactobacillus salivarius
as a prebiotic that regulates chicken intestinal microbiota. Characterization of EPS obtained from
L.
salivarius BIS312 (EPSBIS312) and BIS722 (EPSBIS722) strains was demonstrated by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR), and size-exclusion chromatography (SEC) analyses. It was determined that the molecular weight of both EPS is in the range of 10
4
–10
6
Daltons, and there are 4 types of monomers in their structure. Anti-biofilm and anti-quorum sensing effects of EPSBIS312 and EPSBIS722 were determined. EPSBIS312 and EPSBIS722 showed a strong anti-biofilm effect on
Enterococcus faecalis
ATCC 29212,
Staphylococcus aureus
EB-1, and
Escherichia coli
ATCC 11229. The anti-quorum sensing study revealed that the EPSBIS722 had a higher effect than the EPSBIS312. The effect of different concentrations of EPS (2.5%, 5%, 10%) on
lactobacilli
growth stimulator (LGS) was evaluated. The highest LGS was promoted at 10% concentration while the lowest LGS was promoted at 2.5% concentration by EPSBIS722. In addition, adhesion abilities of EPSBIS312 and EPSBIS722 in HT-29 colorectal adenocarcinoma cell line were tested. EPSs significantly increased the ability to adhere to HT-29 cells. The characterized EPSs may be an alternative to plant prebiotics such as inulin at poultry.